Abstract

Understanding the thermodynamic principles governing surface modifications of biomolecules is crucial for elucidating their behavior and interactions in biological systems. In this study, we investigate the thermodynamics of bio-molecule surface changes through a comprehensive analysis combining experimental techniques and theoretical modeling. By employing surface-sensitive spectroscopic methods, such as surface plasmon resonance (SPR) and quartz crystal microbalance (QCM), we probe the adsorption and desorption processes of biomolecules onto/from various surfaces. Additionally, molecular dynamics simulations are utilized to provide atomistic insights into the energetics and kinetics of bio-molecule interactions with surfaces. Our findings shed light on the thermodynamic driving forces underlying bio-molecule surface modifications, including factors such as surface chemistry, molecular conformation, and environmental conditions. This research contributes to the fundamental understanding of biomolecular behavior at interfaces and has implications for a wide range of fields, including biotechnology, biomaterials science, and drug delivery.