Abstract

Enzyme-linked immunosorbent assay (ELISA) has long been a cornerstone technique in molecular biology, enabling the sensitive and specific detection of proteins in biological samples. Recent advancements in assay design have led to the development of multiplexed ELISA platforms capable of simultaneously measuring multiple analytes within a single sample. This abstract discusses the principles, applications, and benefits of multiplexed ELISA for comprehensive molecular profiling. By leveraging the specificity of antibody-antigen interactions and the sensitivity of enzymatic detection, multiplexed ELISA offers researchers the ability to characterize complex biological systems with high throughput and precision. The versatility of multiplexed ELISA allows for the simultaneous quantification of cytokines, growth factors, signaling molecules, and other biomarkers, facilitating comprehensive molecular profiling in diverse research areas such as cancer biology, immunology, and drug discovery. Furthermore, multiplexed ELISA enables the study of biomarker panels and protein networks, providing valuable insights into disease mechanisms, biomarker discovery, and therapeutic interventions. Overall, multiplexed ELISA represents a powerful tool for elucidating the molecular underpinnings of biological processes and advancing our understanding of health and disease.