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Hindi Special Issue Article
Inhibition of Cell Growth by Nuclear Receptor COUP-TFI: Possible Involvement of Decorin in Growth Inhibition
| Shinji Morii1,2, Masaki Kato3, Naohiko Seki3, Natsuko Shinmen1, Katsuro Iwase1, Masaki Takiguchi1 and Takaki Hiwasa1* | |
| 1Department of Biochemistry and Genetics, Chiba University Graduate School of Medicine, Japan | |
| 2Department of Medicine and Clinical Oncology, Chiba University Graduate School of Medicine, Japan | |
| 3Department of Functional Genomics, Chiba University Graduate School of Medicine, Japan | |
| *Corresponding Author : | Takaki Hiwasa Department of Biochemistry and Genetics Chiba University, Graduate School of Medicine Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan Tel: +81-43-226-2541 Fax: +81-43-226-2037 E-mail: hiwasa_takaki@faculty.chiba-u.jp |
| Received August 05, 2014; Accepted August 07, 2014; Published August 14, 2014 | |
| Citation: Morii S, Kato M, Seki N, Shinmen N, Iwase K, et al. (2014) Inhibition of Cell Growth by Nuclear Receptor COUP-TFI: Possible Involvement of Decorin in Growth Inhibition. Biochem Physiol S4:001. doi:10.4172/2168-9652.S4-001 | |
| Copyright: © 2014 Hiwasa T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
Abstract
The transcription factor, Chicken Ovalbumin Upstream Promoter-Transcription Factor I (COUP-TFI) is a member of the steroid/thyroid hormone receptor superfamily and plays important roles especially in development and differentiation. To investigate the effects of COUP-TFI on cell growth, we transfected this factor stably into Ha-rastransformed NIH3T3 (ras-NIH3T3) cells. Ectopic expression of COUP-TFI resulted in reduction of both proliferation rates and anchorage-independent growth. Flow cytometric analysis showed that progression through mid-S to G2/M phase was delayed in COUP-TFI transfectants. cDNA microarray analysis was performed to elucidate candidate genes whose expression levels were changed in response to COUP-TFI introduction. mRNA levels of decorin and cyclin G were elevated in COUP-TFI transfectants. Luciferase reporter assay revealed that the upstream promoter regions between –252 and -205 bp and between -204 and +42 bp of the decorin gene were responsible for COUPTFI. These suggest that decorin transactivated by COUP-TFI is one of the molecules which account for the growthinhibitory effects of COUP-TFI.
