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Hindi Research Article
In Vitro Test and Molecular Docking of Alkaloid Compound in Marine Sponge Cinachyrella anomala against T47D Cell Cycle
Awik Puji Dyah Nurhayati1*, Rarastoeti Pratiwi2, Subagus Wahyuono3, Istriyati2, Hari Purnomo4 and Syamsudin Abdillah5
1Biology Department, Mathematic and Natural Science Faculty, Sepuluh November Institute of Technology Surabaya, Indonesia
2Biology Faculty, GadjahMada University, Yogyakarta, Indonesia
3Department of Biological Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia
4Department of Chemistry Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia
5Department of Pharmacology, Faculty of Pharmacy, Pancasila University, South Jakarta, Indonesia
- *Corresponding Author:
- Awik Puji Dyah Nurhayati
Biology Department, Mathematic and Natural Science Faculty
Sepuluh November Institute of Technology Surabaya, Indonesia
Tel: +1-031-596-3857 (extn. 123)
Fax: +1-031-596-3857
E-mail: awiknurhayati@gmail.com
Received date February 26, 2015; Accepted date March 28, 2015; Published date March 30, 2015
Citation: Nurhayati APD, Pratiwi R, Wahyuono S, Istriyati, Purnomo H, et al. (2015) In Vitro Test and Molecular Docking of Alkaloid Compound in Marine Sponge Cinachyrella anomala against T47D Cell Cycle. J Marine Sci Res Dev 5:158. doi:10.4172/2155-9910.1000158
Copyright: © 2015 Nurhayati APD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The compound 1,4,9-triazatricyclo[7,3,1,0]trideca-3,5(13),10-trien-8-ol (SA2014) was isolated from the marine sponge Cinachyrella anomala. In vitro assay for SA2014 compound was found to be able to induce cell-cycle arrest at the sub-G1 and G2/M phases of T47D cancerous cell. A combined dosage between of SA2014 compound and of doxorubicin was able to induce cell-cycle arrest at sub-G1 and G2/M phases. Molecular docking approach showed that SA2014 compound inhibited cdk2 enzyme. The strength of interaction between SA2014 and cdk2 (docking score = -65,43) was more stable than the interaction between doxorubicin and cdk2 (-36,59).
