Journal of Biotechnology & Biomaterials
Open Access

Research Article

Hyperdry Human Amniotic Membrane (HD-AM) is Supporting Aciclovir Included Device of Poly-N-p-Vinyl-Benzyl-D-Lactonamide (PVLA) Sphere for Treatment of HSV-1 Infected Rabbit Keratitis Model

Motonori Okabe^1*, Toshiko Yoshida¹, Michiko Suzuki², Mitsuaki Goto³, Masahiro Omori², Masato Taguchi⁴, Ayaka Toda¹, Takuma Suzuki⁵, Kazuaki Nakagawa², Fumitaka Hiramoto², Toyohiko Ushijima², Hiroki Waki⁶, Etsuko Furuichi¹, Kenichi Arai⁷, Kaixuan Zhou¹, Moustafa Fathy Omar⁸, Makoto Nakamura⁷, Yoshihiro Nomura⁵, Toshio Kasama², Kiyoshi Katou⁶, Shigeru Saito⁹and Toshio Nikaido¹

¹Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Japan

²The Research and Development Division, TOA Pharmaceuticals Co., Ltd., Japan

³Molecular Engineering Institute, Kinki University, Japan

⁴Department of Clinical Pharmacokinetics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Japan

⁵Applied Protein Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan

⁶Department of Obstetrics and Gynecology, Takaoka City Hospital, Japan

⁷Department of Life Sciences and Bioengineering, Graduate School of Science and Engineering for Research (Engineering), University of Toyama, Japan

⁸Biochemistry Department, Faculty of Pharmacy, Minia University, Egypt

⁹Department of Obstetrics and Gynecology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Japan

*Corresponding Author:

Okabe M
Department of Regenerative Medicine
Graduate School of Medicine and Pharmaceutical Sciences
University of Toyama, Sugitani 2630, Toyama City
Toyama Pref., 930-0194, Japan
Tel: +81-76-434- 7212
Fax: +81-76-434-5011
E-mail: okabe@med.u-toyama.ac.jp

Received date: November 09, 2016; Accepted date: December 23, 2016; Published date: December 30, 2016

Citation: Okabe M, Yoshida T, Suzuki M, Goto M, Omori M, et al. (2016) Hyperdry Human Amniotic Membrane (HD-AM) is Supporting Aciclovir Included Device of Poly-N-p-Vinylbenzyl-D-Lactonamide (PVLA) Sphere for Treatment of HSV-1 Infected Rabbit Keratitis Model. J Biotechnol Biomater 6:251. doi: 10.4172/2155-952X.1000251

Copyright: ©2016 Okabe M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

The human amniotic membrane (AM) has been widely used in ophthalmic surgery, including in ocular surface reconstruction. However, whether AM is effective for the release of impregnated drugs is unknown. Hyperdry-AM (HD-AM), glutaraldehyde cross-linked HD-AM (Glu HD-AM) and a solvent of poly-N-p-vinylbenzyl- D-lactonamide (PVLA) did not cause irritation to the rabbit eye. Although the concentration of residual glutaraldehyde in Glu HD-AM was approximately 40 ppm (dry-weight basis), no eye irritation was observed. Acyclovir (ACV) containing PVLA-bearing spheres (ACV/PVLA) was loaded into HD-AM (ACV/PVLA HD-AM), and then the therapeutic efficacy of ACV/PVLA HD-AM was compared with that of ACV/PVLA solution in a herpes simplex virus-1 (HSV-1) eye infection model. The former was more effective against ocular infection, and its efficacy was dose- and volume-dependent. Thus, ACV/PVLA HD-AM sheets were very effective as a curative treatment for eye infection with HSV-1. HD-AM can be function as drug delivery system. In the future, it may be possible to combine HD-AM and PVLA in a device that can control the release of hydrophobic medicines.

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