ISSN: 2476-2024

Diagnostic Pathology: Open Access
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  • Research Article   
  • Diagnos Pathol,
  • DOI: 10.4172/2476-2024.8.S13.004

Host Specific Eimeria Genus Diagnosis and QPCR Development in Sheeps and Goats

Shijie Li1, Yichen Jian2, Kaihui Zhang1, Xiaoying Li1, Rongjun Wang1, Longxian Zhang1 and Fuchun Jian1*
1Department of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China
2Department of Animal Science and Technology, Guangxi University, Nanning 530004, China
*Corresponding Author : Dr. Fuchun Jian, Department of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China, Email: jfchun2008@163.com

Received Date: Jun 08, 2023 / Accepted Date: Jul 10, 2023 / Published Date: Jul 10, 2023

Abstract

Coccidiosis of sheep and goats is caused by protozoa in the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. Routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a real-time PCR (qPCR) technique for the diagnosis of Eimeria spp. in sheep and goats. For this purpose, the 18S rRNA region of the DNA of these parasites was selected because it is a region with low variability among Eimeria spp. The qPCR technique was developed using SYBR Green, resulting in a PCR with high sensitivity, and the ability to amplify samples containing only one oocyst of an Eimeria spp. There was no cross-reaction with other intestinal protozoa, such as Blastocystis, Microsporidia, Cryptosporidium, and Giardia duodenum. The repeatability test showed that the coefficient of variation was less than 2%. This indicated that this method has good sensitivity, specificity, and reproducibility. Thus, the feasibility of using qPCR in the diagnosis of the genus Eimeria was demonstrated in this study. This technique was less laborious and required less skill and diagnostic training compared with the micromorphometry technique conventionally used for this diagnosis

Keywords: 18S rRNA; Sheep; Goat; Coccidiosis; Eimeria; qPCR

Citation: Li S, Jian Y, Zhang K, Li X, Wang R, et al. (2023) Host Specific Eimeria Genus Diagnosis and qPCR Development in Sheep and Goats. Diagnos Pathol Open S13:004. Doi: 10.4172/2476-2024.8.S13.004

Copyright: © 2023 Li S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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