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Hindi Research Article
High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake
| Saroj Kumar Sah*, Ajinder Kaur and Jagdeep Singh Sandhu | ||
| School of Agricultural Biotechnology, Punjab Agricultural University, India | ||
| Corresponding Author : | Saroj Kumar Sah School of Agricultural Biotechnology Punjab Agricultural University, India Tel: +91-9779569724 E-mail: saroj-biotec@pau.edu, saroj1021@gmail.com |
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| Received April 22, 2014; Accepted May 28, 2014; Published June 09, 2014 | ||
| Citation: Sah SK, Kaur A, Sandhu JS (2014) High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake. J Rice Res 2:125. doi: 10.4172/jrr.1000125 | ||
| Copyright: © 2014 Sah SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
A new and rapid protocol for production of embryogenic callus and plant regeneration has been reported in Oryza sativa cv. Kitaake. Callus cultures were established from seeds of Kitaake on MS medium supplemented with 2,4-D (3.0 mg/l), BAP (0.25 mg/l) and proline (0.6 g/l) gelled with phytagel (3.0 g/l), after 9 days of seed culturing. The embryogenic calli were regenerated on regeneration medium, i.e. MS medium supplemented with BAP (3.0 mg/l) and NAA (0.2 mg/l) and gelled with agar (8.0 g/l) in combination with phytagel (2.0 g/l) and regeneration occurred in 18 days. The regeneration percentage achieved was very high i.e 82.66% on the above medium. Rooting was achieved on half strength MS medium. The plantlets were hardened and transferred to soil in earthen pots. The regeneration protocol so developed through callus formation was highly reproducible. The plants showed normal growth and flowering under glass house conditions as well as in field conditions.
