Abstract

The present study was conducted to evaluate the effect of an insecticide chlorpyrifos on blood parameters of fish, for which Grass carp (Ctenoparyngdon idella) was been selected as experimental object in lab. Fishes were been divided into two groups one was as control and other was treated group. Fishes to be treated were classified into fish 1, fish 2, and fish 3 to be treated for 3, 6, 9, 12 and 15 days with chlorpyrifos and blood samples were collected from all and analyzed in the lab respectively.

The mean value of different blood parameters for three fish of control group were like for Hemoglobin 4.40 Gdl, RBC .92 s (x103μl-1), HCT9.08%, MCV 101.03%, MCH 47.87 pg-1 MCHC 47.33 Gdl-1, TLC 8100.00 (cmm), Neutrophils 10.0%, Lymphocytes 76.67%,Eosinophils 5.0%, Monocytes 8.3%, Basophils 00.0% and platelets 28000.0(cmm) while mean values of these parameters for three fishes treated with chlorpyrifose for 3 were 5.6 Gdl- 1, 1.04 s (x103μl-1),11.13%, 109.3 %,55.13 pg-1, 50.66 Gdl-1, 8400(cmm), 73%, 7%, 9.66%, 0.00, 17300(cmm), for 6 days were 11.166 Gdl-1, 2.49, 2.9(x103μl-1), 30.96%, 124.4%3, 44.833 , 36, 68, 400, 2.33, 89.66, 3.33, 4.66, 0, and 8 800, for 9 days were 7.866 Gdl-1, 1.49 s (x103μl-1), 17, 112.33, 52.6, 46.66, 21566.67, 9.33, 73.33, 6.33, 11, 0 and 9000, for 12 days were 8.366 Gdl-1), 1.456 s (x103μl-1), 17.1, 118.03, 57.5, 49, 28000, 9, 72, 5, 11, 0 and 19333.3 and for 15 days were 10.2 Gdl-1, 5.303 s (x103μl-1), 22.33, 83.5, 38.5, 46, 33266.67, 9.333, 71.333, 6, 13.33,0 and 13,000 respectively.

By comparison of treated group with control group of fish it is been conclude that insecticides like chlorpyrifos has a vital effect on blood parameters of fish which permit these parameters to show deviation from normal values.