Abstract

This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, by combining cytogenetics and molecular biology, GISH enables researchers to visualize and analyze the structure and behavior of plant chromosomes, leading to valuable insights in horticultural science. In this article, we will explore the applications of Genomic In Situ Hybridization in the realm of horticulture and its significant contributions to understanding and improving plant genomes.