Abstract

Flow cytometry is a powerful analytical technique that revolutionizes the field of single-cell analysis. By combining principles of fluid dynamics, optics, and fluorescence detection, flow cytometry enables simultaneous measurement and characterization of multiple cellular properties at the single-cell level. This article provides an overview of flow cytometry, including its principles, instrumentation, and applications. It explores the diverse applications of flow cytometry in immunology, haematology, cancer research, microbiology, and stem cell analysis. The versatility and advancements in flow cytometry continue to drive its widespread use and contribute to our understanding of complex biological systems.