Research Article
Fed-Batch Bioconversion of Glycerol to 1,3-PD by Using Immobilized Citrobacter freundii Cells
Malgorzata Mizielinska*, Urszula Kowalska, Magdalena Labuda, Joanna Furgala and Artur BartkowiakWest Pomeranian University of Technology, Szczecin, Poland
- Corresponding Author:
- Malgorzata Mizielinska
Faculty of Food Sciences and Fisheries
West Pomeranian University of Technology
Center of Bioimmobilisation and Innovative Packaging Materials
Janickiego 35, 71-270 Szczecin, Poland
Tel: 0048914496132
E-mail: Malgorzata.Mizielinska@ zut.edu.pl
Received date: June 06, 2015; Accepted date: August 13, 2015; Published date: August 20, 2015
Citation: Mizielinska M, Kowalska U, Labuda M, Furgala J, Bartkowiak A (2015) Fed-Batch Bioconversion of Glycerol to 1,3-PD by Using Immobilized Citrobacter freundii Cells. J Biotechnol Biomater 5:193. doi:10.4172/2155-952X.1000193
Copyright: © 2015 Mizielinska M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
This work was especially concerned with the effectiveness of immobilization of C.freundii strain on surface of PU foams (PUFs) modified by peanut shells. The aim of the work was to check the efficiency of immobilization process in 1,3-PD production in comparison to 1,3-PD bioconversion by using free bacterial cells. The results of the study showed that the immobilized C.freundii cells were more efficient than the free culture was. It is the thesis of the present work that reiterated a fed-batch bioconversion by using immobilized strain could be not so time-consuming and not as an expensive as a conversion in bioreactor containing free cells was. The immobilization was also important because the cells attached to the materials became more adapted to the environment, and could be used many times for the conversion of 1,3-PD. Based on the results presented in the study, it could be concluded that the immobilization could help to decrease the cost of 1,3-PD synthesis and shorten the time of the process.