Abstract

We investigated the medicine mechanism of we have a tendency tondanDecoction (WDD) in treating ischemic stroke (IS)using network material medical and molecular arrival strategies. The organic phenomenon Omnibus database was searched with “stroke” as keyword, species as“Homosapiens,” andGeneChip information was used to establish, screen, and analyze the differentially expressed genes of IS.The potential active elements and connected WDD target proteins were screened from the normal Chinese medication systems material medical information. Cytoscape (3.8.2) was accustomed construct the drug-disease-target restrictive network, and Biogenetic and therefore the Cyto NCA plug-in were accustomed construct the protein-protein interaction (PPI). Sequence metaphysics and therefore the urban center reference of Genes and Genome enrichments analyses were performed and Auto Dock was used for molecular arrival verification.

181 active compounds in WDD produced 2497 total targets; of which 8 were linked targets. PPI network showed that JUN, NFKBIA, FOS, and CDKN1A have high sequence node degree values, with JUN exhibiting the largest price. This suggested that JUN perhaps major potential target for WDD treatment of IS. Enrichment analysis showed that WDD core targets were concerned within the IL-17 and tumor necrosis factor signal pathway, lipide and arteriosclerosis, NF kappa B, toll-like receiver, and nod-like receiver signal pathways. Configuration analysis showed that the key elements of WDD were luteolin, naringin, ligustrazine, beta sit sterol, quercetin, and stigmasterol; eachplayed a key role and should serve ascore compounds for treatingIS. Molecular arrival results showed that the active elements in WDD bind well with key targets.

Our results counsel that varied active elements of WDD play a therapeutic role in IS by regulation multiple targets like JUN, NFKBIA, FOS, and CDKN1A in multiple channels like inflammation, immunity, and lipide metabolism.