Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Research Article
Exploited Application of Bacillus spp. ETL-1979 for Degradation and Decolorization of Methyl Orange, Malachite Green and Congo Red
| Maulin P Shah1*, Kavita A Patel1, Sunu S Nair1, A M Darji1 and Shaktisinh Maharaul2 | |
| 1Industrial Waste Water Research Laboratory, Applied and Environmental Microbiology Lab, Enviro Technology Limited (CETP), Gujarat, India | |
| 2Laboratory of Environmental Bioremediation, Narmada Clean Tech Limited (FETP), Ankleshwar, Gujarat, India | |
| Corresponding Author : | Maulin P Shah Industrial Waste Water Research Laboratory Applied and Environmental Microbiology Lab, Enviro Technology Limited (CETP) Plot No: 2413/2414, GIDC, Ankleshwar- 393 002, Gujarat, India Tel: +91-90 999 65504 Fax: +91-2646-250707 E-mail: shahmp@uniphos.com |
| Received: June 15, 2013; Accepted: July 29, 2013; Published: July 31, 2013 | |
| Citation: Shah MP, Patel KA, Nair SS, Darji AM, Maharaul S (2013) Exploited Application of Bacillus spp. ETL-1979 for Degradation and Decolorization of Methyl Orange, Malachite Green and Congo Red. J Bioremed Biodeg 4:196. doi:10.4172/2155-6199.1000196 | |
| Copyright: © 2013 Shah MP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
Related article at Pubmed Scholar Google |
|
Abstract
The local industrial dye waste water sample was collected from Final Effleunt Treatment Plant (FETP) of Ankelshwar, Gujarat, India for the isolation of dye degrading bacteria. The isolate ETL-1979 was selected based on its maximum decolorization of dyes Methyl orange, Congo red and Malachite green (each 150 mg/L). Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence confirmed ETL-1979 as Bacillus sp. On physiochemical parameters optimization, Bacillus sp. ETL-1979 degraded 10.13% (0.15 mg/mL), 11.76% (0.11 mg/ mL) and 9.32% (0.8 mg/mL) of Methyl orange, Congo red and Malachite green, respectively within 2 h, whilst 100% degradation (2.97 mg/mL) at 18 h for Methyl orange, 100% (2.43 mg/mL) at 16 h for Congo red and 100% (3.18 mg/mL) at 20 h for Malachite green were recorded. The degraded products were found non-toxic in nature, while bacterial cells showed elongation and membrane rupturing under SEM taken from dye degraded media.
