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Hindi Research Article
ESTIMATION OF ANTIMICROBIAL AND PHYTOCHEMICAL ACTIVITY OF CLITORIATERNATEA STEM AND LEAF EXTRACTS AGAINST SOME SELECTED BACTERIAL STRAIN
Abstract
Focus on plant research has increased all over the world and there areclear evidencesofmedicinal plants beingused in various traditional remedial systems. Antibacterial evaluation of methanolic, petroleum ether and chloroform extract of leaf and stem of Clitoriaternatea plant was carried out using the disk and well diffusion method.These were tested against two bacteria, Gram positive: Bacillus subtilis, Staphylococcus aureus; Gram negative bacteria: Escherichia coli, Pseudomonas aeroginosa. These leaves and stem extracts exhibited anti-microbial activities with zones of inhibition ranging from 1.2 – 2mm in leaves and 0.5 – 1.5mm stem of methanol and petroleum ether in disk diffusion and in well diffusion bacteria.Bacillus subtilis showed the maximum zone of inhibition (2.5mm)in stems and leaves in methanol extract. Qualitative phytochemical analysis of this plant confirms the presence of various secondary metabolites tannins, carbohydrates, proteins, flavanoids, reducing sugars, glycosides and resins. Methanol was only present in all secondary metabolites. Hence Clitoriaternatea can be used to discover& develop natural products in the new pharmaceuticals and can be effectively used to cure numerous infectious diseases.
