ISSN: 2161-0460

Journal of Alzheimers Disease & Parkinsonism
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  • Research Article   
  • J Alzheimers Dis Parkinsonism 2017, Vol 7(5): 383
  • DOI: 10.4172/2161-0460.1000383

Enhancement of Striatal Dopaminergic Function Following Autologous Neural Cell Ecosystems (ANCE) Transplantation in a Non-Human Primate Model of Parkinson�s Disease

Simon Borgognon1#, Jérôme Cottet1#, Véronique Moret1, Pauline Chatagny1, Nathalie Ginovart2, Cristian Antonescu3, Jocelyne Bloch4, Jean-François Brunet5, Eric M Rouiller1* and Simon Badoud1
1Department of Medicine, Laboratory of Neurophysiology of Action and Hearing, Research cluster Neurosciences, Swiss Primate Competence Center for Research (SPCCR), University of Fribourg, Fribourg, Switzerland
2Department of Psychiatry, University of Geneva, Geneva, Switzerland
3Fribourg Cantonal Hospital (HFR), Nuclear Medicine Unit, , Fribourg, Switzerland
4Department of Neurosurgery, , Lausanne University Hospital (CHUV), Lausanne, Switzerland
5Cell Production Center (CPC), Lausanne University Hospital (CHUV), , Lausanne, Switzerland
#Contributed equally to this work
*Corresponding Author : Eric M Rouiller, Department of Medicine, Laboratory of Neurophysiology of Action and Hearing, Research cluster Neurosciences, Swiss Primate Competence Center for Research (SPCCR), University of Fribourg, Fribourg, Switzerland, Tel: +41 26 300 86 09, Email: eric.rouiller@unifr.ch

Received Date: Sep 26, 2017 / Accepted Date: Oct 05, 2017 / Published Date: Oct 12, 2017

Abstract

Objective: Previous evidence was provided that parkinsonian monkeys exhibited significant though incomplete behavioral recovery following a cell therapy consisting of auto-transplantation of adult neural progenitor cells. The aim of the present study was to assess for the first time in this parkinsonian non-human primate model the striatal dopaminergic function, in parallel to further behavioral assessment. In other words, is the behavioral recovery associated to a reversal of dopaminergic function despite the auto-transplanted cells are not dopaminergic. Methods: Striatal dopaminergic function and motor behavior (spontaneous motion activities) were monitored in adult parkinsonian macaques in relation to autologous neural cell ecosystem (ANCE) transplantation. In four MPTP intoxicated macaques, adult progenitor cells derived from cortical biopsies were re-implanted in the same animal after a phase of spontaneous functional recovery. The function of the striatal dopaminergic system was assessed using 18F-DOPA positron tomography imaging and the motor function was quantified. Results: Two parkinsonian animals exhibited severe motor symptoms, which were moderate and transient in two other monkeys. 18F-DOPA striatal uptake decreased by 80% in three animals, consistent with losses of dopaminergic neurons in substantia nigra and reduced striatal density of dopaminergic projections. Six months after autologous transplantation, all animals improved their motor functions. This functional recovery was largely consistent with positron emission tomography results showing some recovery of 18F-DOPA striatal uptake toward baseline value following transplantation. Conclusion: The present data confirm that symptoms are variable across individual parkinsonian monkeys and that autologous neural cell ecosystem transplantation indeed attenuates parkinsonian motor symptoms. Yet the present study provides for the first time evidence in favor of an increase in the striatal dopaminergic activity that correlates with motor recovery in this novel therapeutic approach, although the implanted cells are not dopaminergic.

Keywords: F-DOPA PET scan; MPTP; Macaque monkeys; Cell therapy; Motor behaviour; TH-immunocytochemistry

Citation: Borgognon S, Cottet J, Moret V, Chatagny P, Ginovart N, et al. (2017) Enhancement of Striatal Dopaminergic Function Following Autologous Neural Cell Ecosystems (ANCE) Transplantation in a Non-Human Primate Model of Parkinson’s Disease. J Alzheimers Dis Parkinsonism 7: 383. Doi: 10.4172/2161-0460.1000383

Copyright: © 2017 Borgognon S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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