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Hindi Research Article
Insilico Analysis of cis acting Regulatory Elements CAREs in Upstream Regions of Ascorbate Glutathione Pathway Genes from Oryza sativa
| Saurabh Pandey1,2*, Chinreddy Subramanaym Reddy1, Ubaid Yaqoob3, Yogesh Kumar Negi4, Sandeep Arora5 and Tanushri Kaul1 | |
| 1Plant Molecular Biology Lab, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India | |
| 2Uttarakhand Technical University, Dehradun-248007, India | |
| 3Department of Botany, University of Kashmir, Srinagar-190006, India | |
| 4Department of Basic Sciences, College of Forestry & Hill Agriculture, University of Horticulture & Forestry, Ranichauri, Uttarakhand-249199, India | |
| 5Department of Molecular Biology and Genetic Engineering, C.B.S.H, G.B.P.U, A & T, Pantnagar, Uttarakahand-263145, India | |
| Corresponding Author : | Saurabh Pandey Plant Molecular Biology Lab International Centre for Genetic Engineering and Biotechnology Aruna Asaf Ali Marg, New Delhi-110067, India Tel: +918447869135 Fax: +91-11-26742316 E-mail: saurabhpandey28@gmail.com |
| Received March 18, 2015; Accepted April 22, 2015; Published April 29, 2015 | |
| Citation: Pandey S, Subramanaym Reddy C, Yaqoob U, Negi YK, Arora S, et al. (2015) Insilico Analysis of cis acting Regulatory Elements CAREs in Upstream Regions of Ascorbate Glutathione Pathway Genes from Oryza sativa. Biochem Physiol 4:159. doi:10.4172/2168-9652.1000159 | |
| Copyright: © 2015 Pandey S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
Abstract
CAREs play an important role in plant stress tolerance by interacting with transcription factors and controlling the expression of many stress related potential genes. Recognition of promoters and their regulatory elements is one of the crucial challenges in biotechnology. In this study, we examine cis acting regulatory element in 5’ upstream regions (~1 kb) of Asc-Glu pathway genes such as SOD, APX, MDHAR, DHAR, and GR. The evolutionary relationships amongst these sequences were deciphered using MEGA v. 6.0. The promoter region these genes contain various cis acting regulatory elements such as MBS, DRE/C repeat, W box, HSE, TCA element, LTR, ABRE box, ARE box, Wun and DRE that have significant role in stress tolerance Asc-Glu promoter sequences analysis revealed their specific responsiveness or overlapping in various environment stress and significantly contribute toward plant growth and development.
