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Hindi Research Article
Bacillus cereus Mediated ε-Caprolactam Degradation: An Initiative for Waste Water Treatment of Nylon-6 Production Plant.
| Mehta SK, Panchal PA, Butala BN and Sane SA* | ||
| Department of Biotechnology, Research and Development Centre, Gujarat State Fertilizers and Chemicals Limited, Vadodara-391750, India | ||
| Corresponding Author : | Sane SA Department of Biotechnology Research and Development centre GSFC Ltd., Vadodara 391750, India Tel: +91 9712986358 Fax: +91 0265 2240966 E-mail: sasane@gsfcltd.com |
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| Received May 14, 2014; Accepted June 05, 2014; Published June 09, 2014 | ||
| Citation: Mehta SK, Panchal PA, Butala BN, Sane SA (2014) Bacillus cereus Mediated ε-Caprolactam Degradation: An Initiative for Waste Water Treatment of Nylon-6 Production Plant. J Bioremed Biodeg 5:230. doi:10.4172/2155-6199.1000230 | ||
| Copyright: © 2014 Mehta SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
Present study focuses on isolation, characterization and application of a novel bacterial strain for biological treatment of waste water from Nylon 6 production plant of Gujarat State Fertilizers and Chemicals (GSFC), Vadodara. This microbe was isolated by applying basal synthetic medium containing ε-caprolactam (precursor of Nylon-6 polymer) as a sole source of carbon and nitrogen. It was identified as Bacillus cereus strain YH-10 on the basis of primary characterization followed by 16s ribosomal RNA gene analysis. This strain has very high tolerance for ε-caprolactam (3.5%) compared to other reported ε-caprolactam -degrading microbes. It degrades 91% and 83.3% ε-caprolactam of synthetic media and Nylon-6 Waste water, respectively in 96 hrs. ε-caprolactam degradation was calculated by analyzing unutilized ε-caprolactam concentration in the samples using liquid chromatography-mass spectroscopy (LC-MS/MS) and chemical oxygen demand (COD) analysis. Based on these observations, biological treatment of waste water from Nylon-6 plant is envisaged utilizing minimum resources.
