Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Effects of Unprocessed Versus Vinegar-Processed Schisandra chinensis on the Activity of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 Enzymes in Rats

Lianlin Su^1,2, Lijun Wang², Ping Li², Chunqin Mao^2*, Min Hao² and Tulin Lu^1,2*

¹The Key Laboratory of Chinese Herbal Medicine Processing of Jiangsu Province, Nanjing, 210046, PR China

²College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, PR China

*Corresponding Author:

Chunqin Mao
College of Pharmacy, Nanjing University of Chinese Medicine
Nanjing, 210046, PR China
Tel: 862585811001
E-mail: mcq63@163.com
Tulin Lu
The Key Laboratory of Chinese Herbal Medicine Processing of Jiangsu Province
Nanjing, 210046, PR China
Tel: 02585811835
E-mail: ltl209@163.com

Received Date: August 21, 2017; Accepted Date: August 26, 2017; Published Date: August 29, 2017

Citation: Su L, Wang L, Li P, Mao C, Hao M, et al. (2017) Effects of Unprocessed Versus Vinegar-Processed Schisandra chinensis on the Activity of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 Enzymes in Rats. J Anal Bioanal Tech 8:375. doi: 10.4172/2155-9872.1000375

Copyright: © 2017 Su L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Schisandra chinensis (SC) is a well-known traditional Chinese herbal medicine that has been used in clinical practices for thousands of years. However, the differences between the effects of unprocessed and vinegarprocessed Schisandra chinensis (VSC) on cytochrome P450 (CYP450) activities are poorly understood. To evaluate the differences between processed and unprocessed SC on the metabolism of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 substrates in rats using a cocktail method based on a developed and validated high performance liquid chromatography-mass spectrometry (HPLC-MS) method. Six probe substrates (coumarin (CYP2A6), bupropion (CYP2B6), paclitaxel (CYP2C8), tolbutamide (CY2C9), omeprazole (CYP2C19) and metoprolol (CYP2D6)) were delivered simultaneously into rats treated with single or multiple doses of processed or crude SC extract. The plasma concentrations of the six probes were profiled by HPLC-MS, and their corresponding pharmacokinetic parameters were calculated. Treatment with single or multiple doses of either extract of SC or VSC induced CYP2A6, CYP2B6 and CYP2C9 enzyme activity and inhibited CYP2D6, CYP2C19 and CYP2C8 enzyme activity in rats. Furthermore, the inhibitory or induced effect of multiple doses of SC was more potent after vinegar processing than without vinegar processing. CYP2A6, 2B6 and 2C9 enzyme activity were induced significantly after treatment with multiple doses but not after a single dose. CYP2C19 enzyme activity were inhibited significantly after treatment with multiple doses but not after a single dose. These results provide useful scientific data for the safe clinical application of either extract of SC in combination with other drugs, which should lack the side effects induced by other herb-drug interactions.

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