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Hindi Research Article
Effects of Mesenchymal Stromal Cells on the Neuropathic Pain Induced by Chronic Constriction Injury in Rats
Deniz Genc1*, Noushin Zibandeh1, Yasin Yildiz2, Sezer Aslan2, Faruk Demirtas2, Erdal Karaoz3,4 and Tunc Akkoc1,5
1Department of Pediatric Allergy and Immunology, School of Medicine, Marmara University, Istanbul, Turkey
2School of Medicine, Marmara University, Istanbul, Turkey
3Center for Regenerative Medicine and Stem Cell Research & Manufacturing, Liv Hospital, Istanbul, Turkey
4Department of Histology and Embryology, Istinye University, Istanbul, Turkey
5Department of Sports Health Sciences, Marmara University, Ä°stanbul, Turkey
- *Corresponding Author:
- Deniz Genc
Department of Pediatric Allergy and Immunology, School of Medicine
Marmara University, Istanbul, Turkey
Tel: +905337495534
E-mail: denizdogan2000@gmail.com
Received date: August 28, 2017; Accepted date: September 19, 2017; Published date: September 25, 2017
Citation: Genc D, Zibandeh N, Yildiz Y, Aslan S, Demirtas F, et al. (2017) Effects of Mesenchymal Stromal Cells on the Neuropathic Pain Induced by Chronic Constriction Injury in Rats. J Pain Relief 6:302. doi:10.4172/2167-0846.1000302
Copyright: © 2017 Genc D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Neuropathic pain remains a persistent clinical problem and characterized by mechanical allodynia and heat hyperalgesia. Chronic pain conditions are among the major health problems which are difficult to treat. Bone marrow-derived mesenchymal stromal cells (BMSCs) have generated great interest as an option for cell-based therapy. BMSCs are easy to isolate and expand ex vivo. Clinical studies show that direct injection of BMSCs does not produce side effects as rejection and is well tolerated by the immune system.
Methods: Neuropathic pain model in rats was developed with the ligation of the sciatic nerve. BMSCs were isolated from femur and tibia aspirates of rats and kept in culture media. rBMSCs were injected locally into injured sciatic nerve area of rats and efficiency of the therapy was observed with thermal sensitivity and motor functions for 4 weeks after injection of rBMSCs.
Results: After injection into injured area rBMSCs were located in sciatic nerve tissue. In the present study, we showed that a single systemic local injection (into the lesion site) of rBMSCs reversed pain hypersensitivity in rats after injury and decreased the pain symptoms for 2 weeks and these effects got back in 4 weeks.
Discussion: Our results revealed that single injection of rBMSCs showed relief of pain in short-term follow-up and further booster injection needed for long term prolonged therapeutic approach.
