Abstract

Hind-limb suspension (HS) is a well-established model for simulating microgravity effects on various physiological systems, including the musculoskeletal and reproductive systems. This study investigates the impact of HS on ovarian oxidative phosphorylation (OXPHOS) and cytoskeleton proteins in mice. Using a combination of biochemical assays, proteomics, and immunohistochemistry, we analyzed changes in OXPHOS enzyme activities and cytoskeletal protein expression in mouse ovaries following HS. Our results indicate significant alterations in mitochondrial function, evidenced by changes in OXPHOS enzyme activities and mitochondrial protein levels. Additionally, HS induced remodeling of cytoskeletal proteins, potentially affecting ovarian cell structure and function. These findings provide insights into the molecular mechanisms underlying ovarian adaptations to altered gravitational forces, with implications for understanding reproductive health in spaceflight and terrestrial conditions.