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Hindi Research Article
Effects of Citrulline Combined with Tadalafil on Monocrotaline-Induced Pulmonary Hypertension in Rats Compared with Arginine
| Fuminobu Ishikura1*, Mai Egawa2, Yuri Takano2, Kota Kumagai3, Takashi Suzuki3 and Masahiko Morita3 | |
| 1Department of Health and Nutrition Faculty of Liberal Arts, Osaka Shoin Women’s University, Osaka, Japan | |
| 2School of Allied Health Sciences, Osaka University, Faculty of Medicine, Osaka, Japan | |
| 3Healthcare Products Development Center, Kyowa Hakko Bio Co., Ltd., Ibaraki, Japan | |
| Corresponding Author : | Fuminobu Ishikura, M.D. Department of Health and Nutrition Faculty of Liberal Arts Osaka Shoin Women’s University, 4-2-26, Hishiyanishi Higashi-Osaka, 577-8550, Japan Tel: (+81) 0-6723-8135 Fax: (+81) 6-6723-8135 E-mail: ishikura.fuminobu@osaka-shoin.ac.jp |
| Received: July 06, 2015; Accepted: August 08 2015; Published: August 12, 2015 | |
| Citation: Ishikura F, Egawa M, Takano Y, Kumagai K, Suzuki T, et al. (2015) Effects of Citrulline Combined with Tadalafil on Monocrotaline-Induced Pulmonary Hypertension in Rats Compared with Arginine. A Systematic Review. J Nov Physiother 5:269. doi:10.4172/2165-7025.1000269 | |
| Copyright: © 2015 Ishikura F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Purpose: Tadalafil, phosphodiesterase5 (PDE5) inhibitor, was reported to have a therapeutic effect on pulmonary hypertension (PH) and citrulline is an amino acid to dilate arteries as an extracellular supplement to improve nitric oxide (NO) production. Therefore, it is expected that combination of tadalafil and citrulline exert a stronger effect for PH. The aim of this study is to evaluate the combination effects of tadalafil and citrulline on monocrotaline (MCT) -induced PH in rats compared with the combination effects of tadalafil and arginine.
Methods: We used 4-week-old male SD rats which developed PH by 5-times subcutaneous injection of MCT. We evaluated heart function by echocardiography and calculated the ratio of RV to LV weight of heart before and after 6-times administration per two weeks of saline, tadalafil alone, citrulline alone and tadalafil and citrulline or arginine.
Results: The survival rate of tadalafil and citrulline administration was 91.7%, which was higher than other groups. The ratio of RV to LV weight in a tadalafil group and a tadalafil+citrulline group were significantly lower than other 3 groups.
Conclusion: Combination therapy of tadalafil and citrulline useful to prevent deterioration of pulmonary hypertension and improve survival rate compared with concomitant use of arginine.
