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Hindi Research Article
Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate (APornA) Microcapsules
Yuan-Gang Liu1,2*, Yan Bai1, Shi-Bin Wang1,2*, Ai-Zheng Chen1,2 and Wen-Guo Wu1,2
1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
2Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China
- Corresponding Authors:
- Yuan-Gang Liu
College of Chemical Engineering
Huaqiao University, Xiamen 361021, China
Tel: +86 592 6162326
Fax: +86 592 6162326
E-mail: ygliu@hqu.edu.cn
- Shi-Bin Wang
Institute of Biomaterials and Tissue Engineering
Huaqiao University, Xiamen 361021, China
E-mail: sbwang@hqu.edu.cn
Received date July 17, 2013; Accepted date August 23, 2013; Published date August 25, 2013
Citation: Yuan-Gang L, Bai Y, Shi-Bin W, Ai-Zheng C, Wen-Guo W (2013) Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate (APornA) Microcapsules. J Biomim Biomater Tissue Eng 18:110. doi: 10.4172/1662-100X.1000110
Copyright: © 2013 Yuan-Gang Liu, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.
