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Hindi Research Article
Effect of Seed Treatment on Seed Germination and Seedling Growth Attributes of Yeheb (Cordeauxia edulis) with In-Vitro Conditions
Chanie Derso1,2* and Tileye Feyissa21Department of Dry land Crop Science, Jigjiga University, Jigjiga, Ethiopia
2Institute of Biotechnology, Addis Ababa University, Addis Ababa, Ethiopia
- Corresponding Author:
- Chanie Derso
Department of Dry land Crop Science
Jigjiga University, Jigjiga, Ethiopia
Tel.: +251 920 36 03 77
E-mail: chaniederso@gmail.com
Received date: May 20, 2015; Accepted date: July 10, 2015; Published date: July 17, 2015
Citation: Derso C, Feyissa T (2015) Effect of Seed Treatment on Seed Germination and Seedling Growth Attributes of Yeheb (Cordeauxia edulis) with In-Vitro Conditions J Biotechnol Biomater 5:188. doi:10.4172/2155-952X.1000188
Copyright: © 2015 Derso C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Yeheb (Cordeauxia edulis) is an evergreen shrub in the bean family that produces seeds in semi-arid bush land on sandy soils of Somalia and Ethiopia. It is drought hardy and one of the major sources of food for both animals and humans. It also used as medicine and source of natural dyes for coloring of food and textiles and to build house as it is termite resistance. Even though it is a multipurpose plant and being encouraged by most people, it becomes endangered due to low seed viability and over exploitation. Those problems have to overcome by micro propagation and regeneration process. The pre-condition of those attempts are having adequate seed treatment and germination. Thus, the objective of this study is to know the effect of seed treatment on seed germination and seedling growth Attributes of C. edulis in In-Vitro conditions. Both coated and de-coated seeds were sterilized using different concentrations of 3%, 5%, 10% and 15% and 2-3 drops of Tween-20 for 5 min and cultured on growth regulators-free solid MS medium. Results in this study revealed that sterilization of de-coated seeds of C. edulis with 5% Clorox for 5 min was found to be valuable for both seed germination and seedling growth. As conclusion, it is important for In Vitro germinated seedlings which are essential for further experimental process of micro propagation and regeneration. Besides, it is used to as effort for further research activities by reducing time and cost.
