Research Article
Development of Molecular Identification of Nitrifying Bacteria in Water Bodies of East Kolkata Wetland, West Bengal
Mousumi Saha1*, Agniswar Sarkar2 and Bidyut Bandhophadhyay1 | |
1Department of Biotechnology, Vidyasagar University, West Bengal, India | |
2Department of Biotechnology (Recognized by DBT, Govt. of India), The University of Burdwan, Burdwan, West Bengal, India | |
Corresponding Author : | Mousumi Saha Department of Biotechnology Vidyasagar University West Bengal, India Tel: +91 9830736189 E-mail: mou.cute@gmail.com |
Received: September 17, 2013; Accepted: October 30, 2013; Published: November 07, 2013 | |
Citation: Saha M, Sarkar A, Bandhophadhyay B (2013) Development of Molecular Identification of Nitrifying Bacteria in Water Bodies of East Kolkata Wetland, West Bengal. J Bioremed Biodeg 4:211. doi:10.4172/2155-6199.1000211 | |
Copyright: © 2013 Saha M, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Nitrifying bacteria plays a major role in converting the waste water to valuable renewable resources for the society. Ammonia is the toxic excretory product of most aquatic organisms and nitrite formed by the oxidation of ammonia is also toxic. Ammonia oxidizing bacteria converts ammonia to nitrite and Nitrite oxidizing bacteria convert nitrite to nitrate. Among them, Nitrosomonas sp. and Nitrobacter sp. has been the most widely studied organisms. An advanced molecular technique made it possible to explore the nitrifying bacteria in the environment and to enhance our knowledge of its functioning. In view of this it would be of prime importance for rapid detection of these strains/ isolates using molecular techniques. The present study was undertaken to develop and validate 16S rDNA sequence analysis of Nitrosomonas sp. and Nitrobacter sp. collected from different bheries in East Kolkata Wetland, West Bengal.