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Hindi Research Article
Development of HPTLC Method for Determination of Amlodipine Besylate and Olmesaratan Medoxomil Using Human Plasma by Liquid Liquid Extraction
Ambadas. R Rote* and Sadhana.K .KandeDepartment of Pharmaceutical Chemistry, M. G. V.’s Pharmacy College, Panchavati, Mumbai - Agra Road, Nashik- 422003, Maharashtra, India
- *Corresponding Author:
- Ambadas.R.Rote
Department of Pharmaceutical Chemistry
M. G. V.’s Pharmacy College, Panchavati
Mumbai - Agra Road, Nashik- 422003, Maharashtra, India
Tel: +91 9579574199
Fax: +91 2532511931
E-mail: roteambadas@gmail.com
Received date: November 13, 2011; Accepted date: December 21, 2011; Published date: December 23, 2011
Citation: Rote AR, Kande SK (2011) Development of HPTLC Method for Determination of Amlodipine Besylate and Olmesaratan Medoxomil Using Human Plasma by Liquid Liquid Extraction. J Anal Bioanal Tech 2:128. doi: 10.4172/2155-9872.1000128
Copyright: © 2011 Rote AR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
A simple, sensitive, rapid and economic high performance thin layer chromatographic method has been developed for determination of amlodipine besylate and olmesaratan medoxomil in human plasma by liquid liquid extraction using paracetamol as an internal standard. The plasma sample was extracted using mixture of methanol: acetonitrile (3.0: 0.1). A concentration range from 500-3000 ng/spot of amlodipine besylate and olmesaratan medoxomil was used for calibration curve respectively. The percent recovery of amlodipine besylate and olmesaratan medoxomil was found to be 90.41 and 90.64 % respectively. The mobile phase consists of chloroform: methanol (9:1, v/v). Densitometric analysis was carried out at wavelength 254 nm. The Rf values for amlodipine besylate, olmesaratan medoxomil and paracetamol was 0.50 ± 0.05, 0.26 ± 0.05 and 0.66 ± 0.05 respectively. The stability of amlodipine besylate and olmesaratan medoxomil in plasma was confirmed during three freeze-thaw cycles at ‘-20°C’, on bench during 24 h and post preparative during 48 h. The proposed method was validated statistically and by performing recovery study for determination of amlodipine besylate and olmesaratan medoxomil in human plasma by liquid liquid extraction.
