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Hindi Research Article
DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LEVOFLOXACIN IN BULK AND TABLET FORMULATION
Abstract
All immediate release tablets are subjected to dissolution studies in 0.1 N HCl as recommended by SUPAC-IR guidelines or in specified dissolution medium as per their official monograph a simple, selective, rapid, and precise double beam UV-Visible spectrophotometer method has been developed and validated for the estimation of Levofloxacin in pharmaceutical dosage form. Levofloxacin is an antibiotic for treating bacterial infections. Many common infections in humans are caused by bacteria. Bacteria can grow and multiply, infecting different parts of the body. Drugs that control and eradicate these bacteria are called antibiotics. Levofloxacin is an antibiotic that stops multiplication of bacteria by preventing the reproduction and repair of their genetic material, DNA. It is in a class of antibiotics called fluoroquinolones. The standard solution of Levofloxacin in 0.1 N HCl showed maximum absorption at 293 nm. Beer-Lambert’s law obeyed in the concentration range of 2-12 μg/ml, with regression, slope and intercept 0.9997, 0.058 and 0.086 respectively. The percentage recovery is between 100±2 which reflect that the method is free from interference of the impurities and other additives during the estimation of drug in formulation. The proposed method can be successfully used for analysis of Levofloxacin in marketed preparations (Livaquin). The results of analysis have been validated statistically and by recovery studies.
