Determination of Tramadol in Liver Tissues Using HPLC-DAD
Received Date: Jan 22, 2018 / Accepted Date: Feb 09, 2018 / Published Date: Feb 13, 2018
Abstract
Tramadol prescribed narcotic analgesic; tramadol overdose was reported old male 35 years. Free tramadol and its metabolite isolated by two methods of extraction, Stas Otto and ammonium sulfate extraction from liver tissues and comparison between efficiency of the two methods. Liver extractions have tramadol and main metabolite O-desmethyltramadol was quantified by HPLC-DAD. Tramadol was determined in liver concentration 27.98 μg/g and 27.93 O-desmethyltramadol in Stas Otto. Liver concentration of tramadol 23.92 μg/g and O-desmethyltramadol 9.62 μg/g in ammonium sulfate extraction. Objective: To determination free tramadol and its metabolite in liver tissues by using two methods of extraction and comparison concentrations by HPLC-DAD of liver old male 35 years over dose in Upper Egypt.
Keywords: Tramadol; Metabolite; Tissues; HPLC-DAD
Citation: Shihata MAA (2018) Determination of Tramadol in Liver Tissues Using HPLC-DAD. J Anal Bioanal Tech 9: 397. Doi: 10.4172/2155-9872.1000397
Copyright: © 2018 Shihata MAA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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