Abstract

The eukaryotic Initiation Factor 2 is a key regulator of protein synthesis in eukaryotic cells, implicated with inside the initiation step of translation. Fertilization of the ocean urchin eggs triggers a fast boom in protein synthesis hobby that is essential for the development into embryonic mobileular cycles. Here we reveal that fertilization triggers eIF2α dephosphorylation, concomitant with a boom in protein synthesis and that induction of the eIF2α phosphorylation is in detail connected with an inhibition of protein synthesis and mobileular cycle arrest. Using a phospho-mimetic protein microinjected into sea urchin eggs, we confirmed that dephosphorylation of eIF2α is essential for protein synthesis hobby and mobileular department development following fertilization. Our consequences reveal that law of eIF2α performs an essential function with inside the protein synthesis upward push that takes place at some stage in early improvement following fertilization.

Keywords: Eukaryotic Initiation; Translation; Phospho-mimetic protein; Fertilization