Abstract

Researchers are looking into the implicit development of natural composites for anticancer remedy. former studies have supposed the cytotoxic effect of helichrysetin towards different cancer cell lines. In this study, we delved the cytotoxic effect of helichrysetin, a naturally being chalcone on four named cancer cell lines, A549, MCF- 7, Ca Ski, and HT- 29, and further illustrated its biochemical and molecular mechanisms in mortal lung adenocarcinoma, A549. Helichrysetin showed the loftiest cytotoxic exertion against Ca Ski followed by A549. Changes in the nuclear morphology of A549 cells similar as chromatin condensation and nuclear fragmentation were observed in cells treated with helichrysetin. Further substantiation of apoptosis includes the instantiation of phosphatidylserine and the collapse of mitochondrial membrane eventuality which are both early signs of apoptosis. These signs of apoptosis are related to cell cycle leaguer at the S checkpoint which suggests that the revision of the cell cycle contributes to the induction of apoptosis in A549. These results suggest that helichrysetin has great capabilities for development as an anticancer agent.