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Hindi Research Article
Degradation of Phenol by Mixed Culture of Locally Isolated Pseudomonas Species
| Sadia Afrin Jame1, A.K.M. Rashidul Alam1, A.N.M. Fakhruddin1* and Md. Khorshed Alam2 | |
| 1Department of Environmental Sciences, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh | |
| 2Institute of Food & Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Ganakbari, Savar, Dhaka 1344, Bangladesh | |
| Corresponding Author : | Dr. A. N. M. Fakhruddin Associate Professor Department of Environmental Sciences Jahangirnagar University Savar, Dhaka-1342 Tel: +8801718217979 E-mail: a.fakhruddin2@mail.dcu.ie |
| Received: August 04, 2010; Accepted: September 14, 2010; Published: September 17, 2010 | |
| Citation: Jame SA, Rashidul Alam AKM, Fakhruddin ANM, Alam MK (2010) Degradation of Phenol by Mixed Culture of Locally Isolated Pseudomonas Species. J Bioremed Biodegrad 1:102. doi:10.4172/2155-6199.1000102 | |
| Copyright: © 2010 Jame SA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Textile, pharmaceuticals and automobile waste most often contain phenolic wastes. An attempt was made to degrade phenol using locally isolated bacteria. Moreover, as in nature pure culture is rarely found rather than mixed culture, therefore, attempt was also made to improve the degradation using mixed culture of Pseudomonas species. All isolates could completely degrade phenol up to 600 ppm. Isolate Pseudomonas FA degraded 800 ppm phenol completely in 72 hours, but the isolates Pseudomonas SA, TK and KA degraded only 39.33, 43.83 and 33.16% of 800 ppm phenol respectively in 96 hours. Complete removal time was also shorter for the isolate Pseudomonas FA compares to the other isolates. Patterns of growth were similar for all of the isolates, but maximum growth was found with the isolate FA on 600 ppm phenol. Complete degradation time was decreased with mixed culture and removal rate of phenol was 25 ppm/h in mixed culture of all combinations and was higher than that of the single culture of the isolates. In mixed culture study, the growth of bacteria was also increased.
