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Hindi Research Article
Decolorization of Synthetic Textile Dye and Enzymes Production by Improved Strains of Pleurotus Species
*Corresponding Author: Singh MP, Centre of Biotechnology, University of Allahabad, Allahabad – 211002, India, Tel: 9415667798, Email: mpsingh.16@gmail.comReceived Date: Mar 01, 2016 / Accepted Date: Oct 25, 2016 / Published Date: Oct 30, 2016
Citation: Singh MP, Srivastava AK (2016) Decolorization of Synthetic Textile Dye and Enzymes Production by Improved Strains of Pleurotus Species. Cell Mol Biol 62:145.
Copyright: © 2016 Singh MP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
In the present investigation the efficiency of three species of white rot fungui - Pleurotus and their improved dikaryons (heterokaryons) was assessed for decolorization of Phenol Red (Phenolsulfonphthalein or PSP) and production of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase enzymes. All the species of Pleurotus i.e. P. flabellatus, P. ostreatus and P. citrinopileatus decolorized PSP well. However, improved dikaryons Pfo 6X9 and Poc 9X6 decolorized the dye more effectively than three species of Pleurotus. The improved dikaryons also showed higher ligninolytic activity than the parental species. Poc 9X6 showed higher LiP (56.14 U), MnP (615.15 U) and Laccase (715.25 U) activity. In the present work different pH, age and concentration of inoculum and effect of surfactant i.e. sodium dodecyl sulphate (SDS) and Tween-80 were analyzed in order to determine the optimum ones to decolorize maximum concentration of dye. 5 ml of 10 days old culture on pH 5.5 and 0.1% Tween-80 supported maximum decolorization of PSP.
