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Hindi Research Article
Cryopreservation of Human Adipose Tissues using Human Plasma
Jong Bin Kim1, Hee Mi Lee1, Seo Yoon Kim1, Kyoung Sik Park2, Jaeman Bae3, Ju Yun Jang1 and Soo-Shin Kim1*1Real Cosmetic Surgery Clinic , Asia Building 580 Sinsa-dong, Gangnam-gu, Seoul 152-892, Korea
2Department of Surgery, Konkuk University School of Medicine, Hwayang-dong, Gwangjin-gu, Seoul, 143-729, Korea
3Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Hwayang-dong, Gwangjin-gu, Seoul, 143-729, Korea
- Corresponding Author:
- Soo-Shin Kim, M.D., Ph.D
Real Department of Plastic Surgery
Asea Building 580 Sinsa-dong
Gangnam-gu, Seoul 152-892, Korea
Tel: 82-2-760-2921
Fax: 82-2-512-1616
E-mail: kim1624@hotmail.com
Received date: April 19, 2011; Accepted date: June 22, 2011; Published date: June 24, 2011
Citation: Kim JB, Lee HM, Kim SY, Park KS, Bae J, et al. (2011) Cryopreservation of Human Adipose Tissues using Human Plasma. J Biotechnol Biomaterial 1:107. doi:10.4172/2155-952X.1000107
Copyright: © 2011 Kim JB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Human adipose tissue or derived cells have been used clinically in several fields. Animal serum is used in storing and culturing human adipose tissue or derived cells. Thus far, few problems have been associated with these sera in current research fields. However, animal sera may cause some problems in clinical applications because of the toxic viruses and proteins that can be present in animal sera. Human plasma can resolve these problems associated with animal serum. In this study, we investigated the effects of human plasma as a cryopreservation solution for human adipose tissues. We observed that adherent cells derived from adipose tissues were isolated effectively by collagenase treatment. We did not observe any adherent cells when human adipose tissues were preserved without cryopreservation solution at ?20°C. Adherent adipose-derived cells exhibited different growth rates based on the cryopreservation solution and storage temperature. We also did not observe adherent cells when human adipose tissues were preserved with cryopreservation solution at ?20°C and ?70°C. Adherent adipose tissue-derived cells exhibited a similar growth rate as cryopreserved tissues when cryopreserved in 10% dimethyl sulfoxide (DMSO) + 90% fetal bovine serum (FBS) or 10% DMSO + 90% human plasma cryopreservation solution but exhibited a low growth rate when cryopreserved in 10% DMSO + 10% FBS + 80% Dulbecco’s modi?ed Eagle’s medium (DMEM) cryopreservation solution. In conclusion, these results demonstrated that human plasma is useful as a cryopreservation solution for human adipose tissues.
