Abstract

The evaluation of Remdesivir in pure form and pharmaceutical dosage formulation has been established and verified using a simple, specific, and repeatable RP-HPLC approach. The estimation was accomplished by Thermo Scientific Hypersil BDS C18 column (150 x 4.6 mm) 5μ i.d. in gradient elution with a mobile phase made up of 10 mM ammonium acetate buffer (pH 3.5 by 2% glacial acetic acid) and acetonitrile, flowing at a rate of 1.0 mL/min. The analyte was measured at a wavelength of 245nm. Remdesivir’s linearity was found to be between 2 and 10 μg/mL, while its recovery was shown to be between 99.01 and 100.04%. The developed method was authenticated as per ICH guideline Q2R2.