Journal of Biotechnology & Biomaterials
Open Access

Research Article

Control of Singular Cell Cycle Synchronization of Mouse ES Cells for Hepatocyte Differentiation on E-Cadherin Substratum

¹Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-57, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan

²Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226,USA

³Frontier Research Center, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan

Corresponding Author:

Toshihiro Akaike
Frontier Research Center, Tokyo Institute of Technology
4259 Nagatsuta-cho, Midori-ku
Yokohama, 226-8503, Japan
Tel: +81-45-924-5790
Fax: +81-45-924-5815
E-mail: takaike@bio.titech

Received date: July 28, 2011; Accepted date: August 25, 2011; Published date: August 27, 2011

Citation: Jovic D, Haque A, Hexig B, Nagaoka M, Akaike T (2011) Control of Singular Cell Cycle Synchronization of Mouse ES Cells for Hepatocyte Differentiation on E-Cadherin Substratum. J Biotechnol Biomaterial 1:113. doi:10.4172/2155-952X.1000113

Copyright: © 2011 Jovic D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Stem cells have enormous potential for therapeutic applications due to their ability to differentiate into diverse cell types. However, preparation of specific lineages at high purity from embryonic stem cells remains a challenge. We have previously reported that embryonic stem (ES) cells on E-cadherin substratum form single-cell scattering morphology. In this study, we report the effect of the hydroxyurea on singular ES cell cycle synchronization to achieve homogeneous population of differentiated cells on E-cadherin substratum. ES cells were successfully arrested in G1 phase with the administration of hydroxyurea and subsequently induced to differentiate into hepatocyte-like cells. The homogeneous population of cells on E-cadherin substratum from synchronized ES cells have higher capability to differentiate into hepatocytes-like cells than unsynchronized ES cells. Moreover, synchronized cells re-enter into the normal cell cycle with the elimination of hydroxyurea for differentiation. Our strategy for ES cell cycle synchronization before differentiation induction possibly helps to increase the yield of hepatocyte-like cells under homogeneous culture condition.

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