Short Communication
Comparative Evaluation between the New BD FACS Count System and Standard BD FACS Count System by Enumeration of Absolute TCD4 Lymphocytes in Adults: Preliminary Results
Zingue D1*, Hien H1,2, Drabo A1, Nouctara M1, Kabore A1, Ouedraogo O1 and Meda N1,3
1 Centre Muraz, Ministry of Health/Burkina Faso, West Africa
2 Institut de Recherche en Sciences de la Santé (IRSS/DRO)/Burkina Faso, West Africa
3 UFR Sciences de la Santé, Université de Ouagadougou/Burkina Faso, West Africa
- Corresponding Author:
- Zingue D
Centre Muraz
Ministry of Health/Burkina Faso
PO Box 390, Burkina Faso, West Africa
Tel: 22620970102
Fax: 22620970457
E-mail: zinguedezemon@yahoo.fr
Received date: December 13, 2013; Accepted date: February 04, 2014; Published date: February 10, 2014
Citation: Zingue D, Hien H, Drabo A, Nouctara M, Kabore A, et al. (2014) Comparative Evaluation between the New BD FACS Count System and Standard BD FACS Count System by Enumeration of Absolute TCD4 Lymphocytes in Adults: Preliminary Results. J Clin Diagn Res 2:103. doi:10.4172/jcdr.1000103
Copyright: © 2014 Zingue D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: The purpose of this study was to evaluate the performance of the new BD FACS Count System compared to the standard BD FACS Count system for count of lymphocytes TCD4.
Methods: It was a comparative study conducted in Centre MURAZ research institute. The New BD FACS Count System dedicated to enumerate absolute and percentage of TCD4+ was compared to the standard BD FACS Count System dedicated to enumerate only absolute number of TCD4+, TCD8+, TCD3+ and the CD4/CD8 ratio. Results were analyzed by Meth Val software.
Results: The New BD FACS Count System compared favorably with the BD FACS Count System for absolute TCD4+, resulting in an overall correlation coefficient of 0.99 for the patients evaluated.
Conclusion: The New BD FACS Count System is simple to perform as the old system and was an excellent alternative method to manage adults HIV in resource limited settings.