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Purpose: Examined in vitro effects of the intracellular zinc chelator, diethyldithiocarbamate acid (DEDTC), zinc
sulphate (ZnSO4) or the combination of both and in vivo effects of DEDTC on retinal taurine system. Qualitative
design was used. A total of 20 participants were purposively selected and semi-structured interviews were
conducted for data collection. Thematic analysis was utilized for data analysis. The research revealed that some
parents do discuss sexual issues and HIV prevention with their adolescents but not a lot. The information that they
discuss is mainly abstinence. The challenges that parents encounter during discussions is lack of skills and
information to initiate the discussion and also cultural beliefs that do not expect a parent to discuss sexual issues
and HIV prevention with their own children.
Methods: Concentrations of DEDTC were 1 to 500 μm and those of ZnSO4 10 and 100 μm for in vitro
experiments. For in vivo assays, DEDTC, 1000 mg/kg, dissolved in saline solution (0.9% NaCl) was administered ip
and the retinal and heart zinc levels were measured at 1, 6 and 24 h after the injection. Levels of zinc in rat retina
and heart were determined in order to establish optimal concentration of DEDTC for further analysis. Zinc was
determined by spectrophotometry. Taurine levels determined by high performance liquid chromatography with
fluorescence detection.
