Advances in Crop Science and Technology
Open Access

Research Article

Characterization of Thioesterase Gene in Sesamum indicum using Bioinformatics Tools

¹Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Andhra Pradesh, India

²Department of Human Genetics, Andhra University, Visakhapatnam, India

*Corresponding Author:

Prakash DSRS
Department of Human Genetics
Andhra University, Visakhapatnam, India
Tel: +9133102078
E-mail: drdevaprakash@gmail.com

Received date: January 16, 2017; Accepted date: February 02, 2017; Published date: February 14, 2017

Citation: Spandana B, Prakash DSRS (2017) Characterization of Thioesterase Gene in Sesamum indicum using Bioinformatics Tools. Adv Crop Sci Tech 5:256. doi: 10.4172/2329-8863.1000256

Copyright: © 2017 Spandana B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Thioesterases play a central role in determining chain lengths of fatty acids in oil storage tissues and have been isolated from a number of plant sources. TEs can be classified into two evolutionarily related families, namely FatA and FatB, with distinct substrate selectivity. With the help of bioinformatics tools alignment was done among the selected thioesterase genes from related oilseed crops. Degenerate primers were designed by manual, codehop and webprimer methods for the conserved regions. Gradient PCR with designed primers showed amplification with manual and codehop primers in sesamum DNA. Amplified DNA is transformed and plasmids are sequenced. Sequences are searched for homology with BLAST. The homology at the amino acid level for thioesterase sequences was observed to be with Helianthus annuus than with the other oil seed crops compared.

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