Abstract

We derived corneal endothelial cell substitute (CECSi) cells from induced pluripotent stem cells (iPSCs) to treat corneal edema caused by endothelial dysfunction (bullous keratopathy) and developed an effective xeno-free protocol to produce CECSi cells from both clinical grade and research grade iPSCs in order to offer regenerative therapy to CECSi cells framed a hexagonal blended monolayer with Na, K-ATPase alpha 1 subunit articulation, tight intersections, N-cadherin adherence intersection development, and atomic PITX2 articulation, which are qualities of corneal endothelial cells. CECSi cells can be cryopreserved, and defrosted CECSi cell suspensions likewise communicated N-cadherin and ATP1A1. In CECSi cells derived from QHJI01s04, the percentage of residual undifferentiated iPSCs was less than 0.01%. Frozen supplies of Ff-I01s04-and QHJI01s04-determined CECSi cells were moved, defrosted and relocated into a monkey corneal edema model. Compared to the control group, corneal edema was significantly reduced in CECSi-transplanted eyes. A promising strategy for providing bullous keratopathy patients with an iPS-cell-based cell therapy to regain useful vision is demonstrated by our findings.