Brief Note on Ultra-High-Performance Liquid Chromatography (UHPLC)
Received Date: Jan 10, 2024 / Accepted Date: Jan 30, 2024 / Published Date: Jan 31, 2024
Abstract
Ultra-High-Performance Liquid Chromatography (UHPLC) represents a significant advancement in chromatographic techniques, offering enhanced speed, sensitivity, and resolution. This brief note provides an overview of UHPLC, highlighting its principles, applications, and key advantages. UHPLC builds upon the foundation of traditional High- Performance Liquid Chromatography (HPLC) with a focus on minimizing particle sizes in the stationary phase and operating at elevated pressures. The use of smaller particles, often below 2 micrometers, coupled with higher pressures, results in improved efficiency and faster separations compared to conventional HPLC. The principles of UHPLC remain rooted in the fundamentals of chromatography, involving a liquid mobile phase and a finely packed column with a highpressure delivery system. The reduced particle size enhances surface area interactions, leading to sharper and more defined chromatographic peaks.
Citation: Ashley N (2024) Brief Note on Ultra-High-Performance LiquidChromatography (UHPLC). J Anal Bioanal Tech 15: 600.
Copyright: © 2024 Ashley N. This is an open-access article distributed under theterms of the Creative Commons Attribution License, which permits unrestricteduse, distribution, and reproduction in any medium, provided the original author andsource are credited.
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