Research Article
Bioremoval of Azo dye Reactive Red by Bacillus spp. ETL-1982
Maulin P Shah*, Patel KA, Nair SS and Darji AM | |
Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP), Plot No: 2413/2414, GIDC, Ankleshwar- 393 002, Gujarat, India | |
Corresponding Author : | Maulin P Shah Applied and Environmental Microbiology Lab Enviro Technology Limited (CETP), India Tel: +91-90 999 65504 Fax: +91- 2646-250707 E-mail: shahmp@uniphos.com |
Received: March 11, 2013; Accepted: April 26, 2013; Published: April 28, 2013 | |
Citation:Shah MP, Patel KA, Nair SS, Darji AM (2013) Bioremoval of Azo dye Reactive Red by Bacillus spp. ETL-1982. J Bioremed Biodeg 4:186. doi:10.4172/2155-6199.1000186 | |
Copyright: © 2013 Shah MP, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Bacillus spp. ETL-1982, with notable ability to decolorize the reactive textile dye Reactive Red, was isolated from the activated sludge collected from a textile mill. Phenotypic characterization and phylogenetic analysis of the 16S rDNA sequence indicated that the bacterial strain belonged to the genus Bacillus. Bacillus ETL-1982 showed a strong ability to decolorize reactive textile dye at pH 9.0 and 37°C and with 4 gL-1 glucose concentrations was considered to be the optimum decolorizing conditions. Bacillus spp. ETL-1982 grew well in a high concentration of dye (300 mgl-1), resulting in approximately 96% decolorization extent in 36 h, and could tolerate up to 1000 mg l-1 of dye. UV–Vis analyses and colorless bacterial cells suggested that Bacillus spp. ETL-1982 exhibited decolorizing activity through biodegradation, rather than inactive surface adsorption. High decolorization extent and facile conditions show the potential for this Bacillus ETL-1982 to be used in the biological treatment of dyeing mill effluents.