Advances in Crop Science and Technology
Open Access

Research Article

Bioinformatic Characterization of Desaturase Partial Gene in Sesamum indicum

¹Institute of Biotechnology (IBT), Acharya NG Ranga Agricultural University (ANGRAU), Guntur, Andhra Pradesh, India

²Department of Human Genetics, Andhra University, Visakhapatnam, Andhra Pradesh, India

*Corresponding Author:

Spandana B
Institute of Biotechnology (IBT)
Acharya NG Ranga Agricultural University (ANGRAU)
Guntur, Andhra Pradesh
India
Tel: 919440327774
E-mail: spandanabandila@gmail.com

Received date: February 10, 2017; Accepted date: February 20, 2017; Published date: February 27, 2017

Citation: Spandana B, Prakash DSRS, Nagalakshmi S (2017) Bioinformatic Characterization of Desaturase Partial Gene in Sesamum indicum. Adv Crop Sci Tech 5: 259. doi: 10.4172/2329-8863.1000259

Copyright: © 2017 Spandana B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

By using Bioinformatic tools three sets of codehop, webprimer and manually, gene specific degenerate primers were designed for Sesamum desaturase. Through Gradient PCR approach maximum homology among desaturase sequences was observed to be with Arachis hypogaea than the other oil seed crops compared. Primers designed through Codehop and webprimershowed amplification of the gene sequence. The aim of this present study was to utilize bioinformatic tools for sesame improvement programs towards diversifying the fatty acid composition of the seed oil.

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