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Hindi Research Article
Assignment of functional groups in Gram-positive bacteria
Boguslaw Buszewski1,2,*, Ewelina Dziubakiewicz1,2, Pawel Pomastowski1,2, Katarzyna Hrynkiewicz3, Justyna Ploszaj-Pyrek4,5, Ewa Talik4, Markus Kramer5 and Klaus Albert5
1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7, 87-100 Torun, Poland
2Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wilenska 4, 87-100 Torun, Poland
3Department of Microbiology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland
4August Chelkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice, Poland
5Department of Biology and Environmental Protection, University of Silesia, Jagiellonska 26/28, 40-032 Katowice, Poland
- *Corresponding Author:
- Boguslaw Buszewski
Chair of Environmental Chemistry
and Bioanalytics, Faculty of Chemistry
Gagarin 7 87-100 Torun, Poland
Tel: (48) (56)6114308
E-mail: bbusz@chem.uni.torun.pl
Received Date:January 27, 2015; Accepted Date February 17, 2015; Published Date February 25, 2015
Citation: Buszewski B, Dziubakiewicz E, Pomastowski P, Hrynkiewicz K, Ploszaj- Pyrek J, et al. (2015) Analytical Method Development and Validation: A Concise Review. JAnal Bioanal Tech 6:232. doi: 10.4172/2155-9872.1000232
Copyright: © 2015 Buszewski B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The chemical composition, molecular structure and physicochemical properties of five Gram-positive bacterial strain: Bacillus cereus, Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Micococcus luteus were investigated by Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), NMR spectroscopy and intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (IC MALDI TOF MS). An analysis of FTIR spectra as a function of pH revealed the three major types of cell wall functional groups – carboxyl group, amino group and phosphate group. An analysis of XPS spectra was determinate the major surface components of bacterial cell. 13C NMR and IC MALDI TOF MS spectra of six bacterial species were registered. Our findings indicate that chemical and structural differences in the cell composition of Gram-positive bacteria can be detected. The obtained results also demonstrate that the combination of FTIR, XPS and NMR spectroscopy with IC MALDI TOF MS technique yields useful information and complements other biochemical and physical methods of microbial cells characteristics.
