Abstract

Microspectrophotometry, a powerful analytical technique combining microscopy and spectroscopy, has revolutionized biological and biomedical research. By facilitating the study of absorbance, fluorescence, and reflectance spectra at the cellular or subcellular level, it provides crucial insights into molecular composition, cellular dynamics, and tissue pathology. This article discusses the fundamental principles and technological advancements in microspectrophotometry. It further elaborates on its wide-ranging applications, including molecular characterization, disease diagnostics, pharmacological research, and the study of environmental impacts on biological systems. Challenges and prospects of this technique in future research contexts are also explored.