Abstract

Glycoproteins are trustworthy biomarkers for detecting cancer in its earliest stages, and prompt treatment in response to these biomarkers may increase survival rates and ease the strain on healthcare systems. Conventional detection methods, such as immune-based assays, on the other hand, frequently require natural antibodies, intricate sample preparations, and lengthy processing durations, which fall short of offering quick turnaround times. In an effort to solve this issue, a Janus gold nanoparticles nanozyme (J-GNPsNE) linked magnetic titanium dioxide molecularly imprinted particles (MTi-MIP) satellite structure immunosandwich colorimetric strategy (SS-ICS) is suggested to for the first time rapidly detect glycoproteins in human serum. The assembly of molecularly imprinted nanoparticles, glycoprotein, and nanozyme in the shape of a sandwich is what gives the method its distinctive detection mode. This made it possible to develop a point-of-care serum glycoprotein biomarkers detection instrument that was laborsaving, simple to use.