Abstract

It is difficult to achieve fM-level viral DNA detection without target amplification. Preamplifying low-abundant DNA targets is a traditional prerequisite that not only lengthens the detection time overall but also raises the risk of infectious material leakage and increases the likelihood of false positive detection. In order to improve the limit of detection (LOD) of this CRISPR-Cas12a detection system to the femtomolar level—three orders of magnitude lower than a standard protocol—we developed a combinatory approach.