Abstract

Recently plenty of success has been done regarding acquisition of mass spectrometric isolation of   differentially  controlled proteins   along  with    invention of     newer proteins, biomarkers, protein  modifications as well as polymorphisms   in different human tissues [1]. However, hurdles  continue    regarding the  assessment in addition to evaluation of properties of proteins existent in lesser quantities inclusive of,   corroborating what are known as  the ‘’missing proteins’’.A missing protein implies a nonverified genetic  sequencing regarding  which  a protein has not been  found till date [2].As  per the International Human Proteome Project(HUPO),at present1343 missing proteins without any function assigned exist, either anticipated by bioinformatics assessment  or by experimental evaluation. The problems encountered regarding estimation of the missing proteins might be associated with their lesser quantities in numerous tissues besides getting expressed in just occasional cell kinds in the human body.