Journal of Clinical Diabetes
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  • Research Article   
  • J Clin Diabetes 2018, Vol 2(2): 102

A Genome-wide Association Study in the Diabetic Patients Finds the 13q35.43-35.46 Locus Associated with Estimated Glomerular Filtration Rate: The Japan Multi-Institutional Collaborative Cohort Study

Asahi Hishida19, Toshiro Takezaki14, Sadao Suzuki15, Miki Watanabe15, Nagato Kuriyama16, Teruhide Koyama16, Norihiro Furusyo17, Hiroaki Ikezaki17, Masahiro Nakatochi18, Sayo Kawai19, Ippei Shimoshikiryo14, Rieko Okada119, Takashi Tamura19, Mariko Naito20, Kenji Wakai19, Yukihide Momozawa21, Michiaki Kubo21, Hirotsugu Ueshima6, Yoshikuni Kita2,22, Megumi Hara7, Akira Narita3, Tsuyoshi Hachiya4, Yoichi Sutoh4, Atsushi Shimizu4, Seiko Ohno5, Naoyuki Takashima2, Harumitsu Suzuki6, Keitaro Tanaka7, Yasuyuki Nakamura1,2*, Kiyonori Kuriki8, Kaori Endoh8, Isao Oze9, Hidemi Ito10,11, Hirokazu Uemura12, Sakurako Katsuura-Kamano12, Haruo Mikami13 and Yohko Nakamura13
1Department of Food Science and Human Nutrition, Ryukoku University, Otsu, Japan
2Department of Public Health, Shiga University of Medical Science, Otsu, Japan
3Department of Preventive Medicine and Epidemiology, Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan
4Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Iwate Medical University, Shiwa-gun, Iwate, Japan
5Department of Bioscience and Genetics, National Cerebral and Cardiovascular Center, Suita, Japan
6Center for Epidemiologic Research in Asia, Shiga University of Medical Science, Otsu, Japan
7Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan
8Laboratory of Public Health, School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
9Division of Cancer Epidemiology and Prevention, Department of Preventive Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan
10Division of Molecular and Clinical Epidemiology, Aichi Cancer Center Research Institute, Nagoya, Japan
11Department of Epidemiology, Nagoya University Graduate School of Medicine, Nagoya, Japan
12Department of Preventive Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan
13Cancer Prevention Center, Chiba Cancer Center Research Institute, Chiba, Japan
14Department of International Island and Community Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
15Department of Public Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
16Department of Epidemiology for Community Health and Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
17Department of Environmental Medicine and Infectious Disease, Kyushu University, Fukuoka, Japan
18Statistical Analysis Section, Center for Advanced Medicine and Clinical Research, Nagoya University Hospital, Nagoya, Japan
19Department of Preventive Medicine, Nagoya University Graduate School of Medicine, Nagoya, Japan
20Department of Maxillofacial Functional Development, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
21Laboratory for Genotyping Development, Center for Integrative Medical Sciences, RIKEN, Yokohama, Japan
22Faculty of Nursing Science, Tsuruga Nursing University, Tsuruga, Japan
*Corresponding Author : Yasuyuki Nakamura, Department of Food Science and Human Nutrition, Ryukoku University, Otsu, Japan, Tel: +81-77-559-5646, Fax: +81-77-559-5646, Email: nakamury@agr.ryukoku.ac.jp

Received Date: Jul 15, 2018 / Accepted Date: Jul 19, 2018 / Published Date: Jul 27, 2018

Abstract

Objective: To conduct a genome-wide association studies (GWAS) to find genetic variations that affected renal function in the diabetic patients in Japan.
Research design and methods: In a Japanese population of 955 patients with type 2 diabetes mellitus (T2D), extracted from 14,091 participants appropriate for GWAS from the Japan Multi-Institutional Collaborative Cohort (JMICC) study. Genotyping was performed at a central laboratory with use of a HumanOmniExpressExome-8 v1.2 BeadChip array. Genotype imputation was conducted with use of SHAPEIT, followed by Minimac3 software (with the 1000 Genomes phase 3 as the reference panel). We calculated the estimated glomerular filtration rate (eGFR) for each patient according to Matsuo et al. Association for the imputed variants with eGFR was performed by linear regression analysis with adjustments for age and sex.
Results: We found 77 SNVs upstream of the NBEA genes that were significantly associated with eGFR in T2D participants with P values <5 × 10-8. This gene was reported as participatory in several metabolic functions and was associated with some disease conditions. However, no previous reports implied that the gene was related to nephropathy in diabetes.
Conclusion:
We found the 13q35.43-35.46 locus upstream of the NBEA gene was significantly associated with eGFR in participants with T2D in a Japanese population.

Keywords: Genome-wide association study; Diabetes mellitus; Estimated glomerular filtration rate; Chronic kidney disease

Citation: Nakamura Y, Narita A, Hachiya T, Sutoh Y, Shimizu A, et al. (2018) A Genome-wide Association Study in the Diabetic Patients Finds the 13q35.43-35.46 Locus Associated with Estimated Glomerular Filtration Rate: The Japan Multi-Institutional Collaborative Cohort Study. J Clin Diabetes 2: 102.

Copyright: © 2018 Nakamura Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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