Outline of Chiral Chromatography
Received: 01-Jun-2023 / Manuscript No. jabt-23-102144 / Editor assigned: 03-Jun-2023 / PreQC No. jabt-23-102144 / Reviewed: 17-Jun-2023 / QC No. jabt-23-102144 / Revised: 19-Jun-2023 / Manuscript No. jabt-23-102144 / Accepted Date: 26-Jun-2023 / Published Date: 27-Jun-2023 QI No. / jabt-23-102144
Abstract
Chiral chromatography is a specialized form of liquid chromatography that focuses on the separation and analysis of enantiomers, which are mirror-image isomers of a molecule. Enantiomers possess identical physical and chemical properties but exhibit distinct biological activities. Chiral chromatography plays a crucial role in various industries, including pharmaceuticals, agrochemicals, and fine chemicals, where the identification and quantification of enantiomers are of great importance. The principle of chiral chromatography lies in the interaction between chiral stationary phases and enantiomers. Chiral stationary phases possess chiral recognition sites that selectively interact with one enantiomer over the other based on their spatial arrangement. This selective interaction drives the separation of enantiomers, leading to their individual identification and quantification. Different modes of chiral chromatography, such as normalphase, reversed-phase, ion-exchange, ligand-exchange, and supercritical fluid chromatography, utilize different types of chiral stationary phases and mobile phases to achieve enantiomeric separation. These modes find applications in pharmaceutical analysis, agrochemical analysis, fine chemical production, and environmental analysis. Advancements in chiral chromatography have expanded its capabilities and improved separation efficiency. Novel chiral stationary phases with enhanced selectivity and stability have been developed. Optimization of mobile phase composition through the incorporation of chiral additives or chiral selectors has improved enantiomeric resolution. The coupling of chiral chromatography with mass spectrometry enables simultaneous separation, detection, and identification of enantiomers. Automation and high-throughput analysis systems have increased the efficiency of chiral chromatographic analysis. Dedicated software tools aid in method development, optimization, and data analysis for chiral separations.
Conclusion: Chiral chromatography is a powerful analytical technique that allows for the separation and analysis of enantiomers. Its applications in various industries and the continuous advancements in chiral stationary phases, mobile phases, and instrumentation have contributed to its importance in the field of stereochemistry. Chiral chromatography plays a crucial role in the development, analysis, and quality control of chiral compounds, providing valuable insights into their biological activities and environmental impacts.
Keywords
Chiral chromatography; Enantiomers; Chiral stationary phase; Chiral recognition; Normal-phase chiral chromatography; Reversed-phase chiral chromatography; Ion-exchange chiral chromatography
Introduction
Chiral chromatography is a specialized form of liquid chromatography that focuses on the separation and analysis of enantiomers, which are mirror-image isomers of a molecule. Enantiomers possess identical physical and chemical properties but exhibit distinct biological activities. Chiral chromatography plays a crucial role in pharmaceutical, agrochemical, and fine chemical industries where the identification and quantification of enantiomers are of paramount importance. This article delves into the principles, applications, and advancements in chiral chromatography.
Principles of chiral chromatography
Chiral chromatography exploits the interaction between chiral stationary phases and enantiomers to achieve their separation. Chiral stationary phases are designed to possess chiral recognition sites that selectively interact with one enantiomer over the other based on their spatial arrangement. The separation is driven by differences in adsorption, partitioning, or bonding interactions between the enantiomers and the chiral stationary phase.
Types of chiral chromatography
There are different modes of chiral chromatography, each utilizing different chiral stationary phases:
Normal-phase chiral chromatography: Utilizes polar stationary phases, such as cellulose or amylose, and non-polar mobile phases.
Reversed-phase chiral chromatography: Employs hydrophobic stationary phases, such as modified silica or cyclodextrins, with polar mobile phases.
Ion-exchange chiral chromatography: Involves ion-exchange stationary phases with charged enantiomers and ionic mobile phases.
Ligand-exchange chiral chromatography: Uses chiral ligands immobilized onto a stationary phase to interact with the enantiomers.
Supercritical fluid chromatography: Utilizes supercritical fluids as the mobile phase with chiral stationary phases.
Materials and Methods
Chiral stationary phase: Chiral stationary phases (CSPs) are the key components of chiral chromatography. They are designed to have chiral recognition sites that interact selectively with enantiomers based on their spatial arrangement. Common types of CSPs include polysaccharides (e.g., cellulose and amylose), cyclodextrins, proteinbased phases, chiral crown ethers, and chiral ligands immobilized onto a solid support. The selection of the appropriate CSP depends on the nature of the enantiomers and the separation mechanism desired [1-3].
Mobile phase: The mobile phase carries the sample through the chiral chromatography system and influences the separation of enantiomers. The choice of mobile phase depends on the type of chiral chromatography being performed (e.g., normal-phase or reversedphase) and the properties of the enantiomers. Common mobile phase components include organic solvents (such as methanol, acetonitrile, or ethanol), water, buffers, and additives (e.g., acids, bases, or chiral selectors).
Chromatography system: Chiral chromatography can be performed using various chromatography systems, including highperformance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), or thin-layer chromatography (TLC). HPLC is the most commonly used technique for chiral separations due to its high resolution, sensitivity, and versatility.
Sample preparation
Sample preparation is an essential step in chiral chromatography to ensure accurate and reliable analysis.
It may involve sample extraction, purification, and derivatization, depending on [1-5] the nature of the analyte and the sample matrix. Sample preparation techniques include solid-phase extraction, liquidliquid extraction, filtration, and concentration.
Method development
Method development in chiral chromatography involves the optimization of various parameters to achieve efficient enantiomeric separation. Parameters to be optimized include the selection of appropriate CSP and mobile phase composition, such as solvent ratio, pH, and the addition of chiral additives or modifiers. Gradient elution or isocratic elution methods can be employed based on the specific separation requirements.
Results and Discussion
Detection
The detection of enantiomers in chiral chromatography is typically performed using various detectors, such as UV-Vis, fluorescence, or mass spectrometry (MS). UV-Vis detectors are commonly used due to their wide availability, sensitivity, and compatibility with most chiral chromatography systems. Mass spectrometry detection provides additional structural information and can be coupled with chiral chromatography to achieve simultaneous separation and identification [6-10].
Data analysis
Data analysis in chiral chromatography involves the quantification and interpretation of chromatographic peaks to determine enantiomeric composition. Calibration curves using enantiomerically pure standards are typically used for quantification. Software tools and algorithms are available to assist in data analysis, peak integration, and enantiomeric purity determination [11].
Advancements in chiral chromatography
Advancements in chiral chromatography have expanded its capabilities and improved separation efficiency:
New chiral stationary phases: The development of novel chiral stationary phases with enhanced selectivity and stability has broadened the range of chiral separations.
Chiral Mobile Phases and Additives: Optimization of mobile phase composition by incorporating chiral additives or chiral selectors has improved enantiomeric resolution.
Hyphenation with mass spectrometry: The coupling of chiral chromatography with mass spectrometry allows for simultaneous separation, detection, and identification of enantiomers [12].
Automation and high-throughput analysis: Automated sample preparation and injection systems have increased the efficiency and throughput of chiral chromatographic analysis.
Method development software: Dedicated software tools assist in method development, optimization, and data analysis for chiral separations.
Chiral chromatography finds extensive applications in various industries:
Pharmaceuticals: Chiral separation is crucial in drug development to assess the safety, efficacy, and pharmacokinetics of enantiomers. Chiral chromatography helps in the analysis of chiral drugs, determination of enantiomeric purity, and separation of drug metabolites.
Agrochemicals: Chiral separation assists in the analysis and characterization of chiral pesticides, herbicides, and fungicides, as well as the determination of enantiomeric ratios, which can have different biological activities and environmental impacts.
Fine chemicals: Chiral chromatography plays a vital role in the production and quality control of enantiomerically pure compounds used in flavors, fragrances, and specialty chemicals.
Environmental Analysis: Chiral separation is employed to determine the fate, behavior, and ecotoxicity of chiral pollutants and their metabolites in environmental samples.
Conclusion
The materials and methods of chiral chromatography encompass the selection of appropriate chiral stationary phases, optimization of the mobile phase composition, sample preparation techniques, method development, detection, and data analysis. The successful implementation of these components ensures efficient separation and accurate analysis of enantiomers in chiral chromatography.
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Citation: Wang M (2023) Outline of Chiral Chromatography. J Anal Bioanal Tech 14: 529.
Copyright: © 2023 Wang M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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