Occurrence and Summary of Literature Documenting Tumors with EWSR1 and CREB Family Transcription Factor Fusions
Received: 25-Jan-2022 / Manuscript No. JCEP-22-52363 / Editor assigned: 27-Jan-2022 / PreQC No. JCEP-22-52363(PQ) / Reviewed: 08-Feb-2022 / QC No. JCEP- 22-52363 / Revised: 15-Feb-2022 / Manuscript No. JCEP-22-52363(R) / Published Date: 23-Feb-2022 DOI: 10.4172/2161-0681.1000407
Abstract
Background: Fusions between the EWSR1 gene and CREB family transcription factors have been reported in a variety of rare tumor types, including the head and neck tumors clear cell odontogenic carcinoma (CCOC) and clear cell carcinoma (CCC) of the salivary gland.
Objective: To review the current literature on tumors where EWSR1-CREB fusions have been reported (i.e., EWSR1-ATF1, -CREB1, -CREM) that may act as surrogates for clear cell odontogenic carcinoma (CCOC) and clear cell carcinoma (CCC) of the salivary gland given their rarity.
Methods: The PubMed database was searched for tumors bearing EWSR1 and CREB family transcription factor fusions. The search was conducted independently by two authors between the publication dates of 1985 and 2021. Search parameters included but were not limited to “EWSR1-ATF1,” “EWSR1-CREB1,” and “EWSR1-CREM.” Data collected included tumor name, EWSR1 translocation partner, histology, immunohistochemical (IHC) markers, presence of clear cells, patient demographics and anatomic location when available.
Results: A total of 103 articles were reviewed and 17 different types of tumors (CCOC and CCC included) were identified. The following 6 were found to consistently bear the same translocations characteristic of CCOC and CCC: angiomatoid fIbrous histiocytoma (AFH), clear cell sarcoma (CCS), clear cell sarcoma-like tumor of the gastrointestinal tract (CCSLTGT), intracranial myxoid mesenchymal tumor (IMMT), malignant gastrointestinal neuroectodermal tumor (M-GNET), and primary pulmonary myxoid sarcoma (PPMS). The remaining 9 tumors were either inconsistently identified or rarely harbored and EWSR1-CREB fusion: angiosarcoma, atypical central neurocytoma, malignant epithelioid tumor of the peritoneal cavity, malignant mesothelioma, neuroendocrine neoplasm, primary intracranial neoplasm, pulmonary mesenchymal tumor, small blue round cell tumor and soft tissue myoepithelial tumor.
Conclusion: EWSR1-CREB fusions have been reported in many tumor types and our review of the literature shows that these fusions are a consistent finding in a smaller set of 6 diagnostic entities in addition to CCOC and CCC. While these tumors exhibit differences in demographics, anatomic location and immunohistochemical profiles, the high frequency of EWSR1-CREB fusions suggests that they may be driven by similar oncogenic mechanisms. Identifying similarities among these rare tumor types may thus be advantageous for gaining insights into their pathogenesis.
Keywords: CCOC; CCS; CCC; EWSR1; CREB1; CREM; ATF1; fusion
Introduction
Ewing sarcoma breakpoint region 1 (EWSR1) is a gene on chromosome band 22q12.2 that encodes an RNA-binding protein and was first identified as a partner in fusions in Ewing sarcoma [1]. The N-terminal disordered domain is responsible for the protein’s transcriptional activation role in the context of gene fusions [2]. Since their initial identification, EWSR1 translocations have been observed in a variety of tumors and has been shown to have several different fusion partners. In fact, Ewing sarcoma is often characterized by the presence of a gene fusion between EWSR1 and members of the ETS family including FLI1, ERG and FEV [3-5]. To date, most tumors with an EWSR1 translocation are sarcomas originating in mesenchymal tissues, with the only known exceptions being CCOC (clear cell odontogenic carcinoma) and CCC (clear cell carcinoma of the salivary gland) [6,7].
The National Cancer Institute defines a rare tumor as a cancer that occurs in fewer than 15 out of 100,000 people each year. Most rare cancers are often more difficult to prevent, diagnose, and treat than the more common ones. The study of rare tumors is difficult due to the lack of availability of fresh tissue. As such, to increase the cohort of cases, surrogates need to be identified based on commonalities in clinical behavior, histopathology, IHC and molecular and genetic transcriptional defects. Our tumor of interest is clear cell odontogenic carcinoma (CCOC) and has only 119 published cases worldwide (1985-2021). Additionally, its intraosseous nature results in many of these specimens having been decalcified. It is also an extremely slow growing tumor making it difficult to grow in the laboratory. A similar tumor, clear cell carcinoma of the salivary gland (CCC, formerly known as hyalinizing clear cell carcinoma), is postulated to be its soft tissue counterpart and is almost as rare with a total of 254 cases reported in the literature [6-8].
Both CCC and CCOC demonstrate comparable clinical behavior, have similar IHC patterns and have translocations involving the EWSR1 gene with translocation partners of ATF1, CREB1 or CREM [7,9]. Eight different teams [6,9-15] have studied the genetics of CCOC. To date, twenty COCC specimens have been tested for EWSR1 translocation using either fluorescence in situ hybridization (FISH) [6,13,15] or RT- PCR and FISH analysis [11], or RNA-seq [9,14]. EWSR1 is shown to be partnered with ATF1 (n=7), CREB1 (n=2), CREM (n=2) [9,14] and, the remainder with an unknown rearrangement location and/or partner. Six of these specimens, however, were negative for an EWSR1 translocation [6,10]. The significance of this is unknown but may reflect limitations of the sequencing technologies used.
Both CCC and CCOC have a preponderance of clear cells [8,16] although the significance of these clear cells is unknown. clear cell sarcoma (CCS) may also be similar to CCOC and CCC. All three tumor types have clear cells and a EWSR1 rearrangement involving partners in the CREB family of transcription factors (ATF1, CREB1 or CREM). Additionally, these tumors appear in soft tissue as well as bone, have a prolonged clinical course and appear to have a slight predilection to women [8,16].
In this study, tumors with the same gene fusions that are found in CCOC (i.e., EWSR1-ATF1, -CREB1, -CREM) were identified and summarized.
Materials and Methods
The databases available through PubMed were used to identify articles describing EWSR1-CREB of transcription factors fusion-fusion-harboring tumors. The PubMed database was searched independently by two authors between the publication dates of 1985 and 2021. Search parameters included but were not limited to “EWSR1- ATF1,” “EWSR1-CREB1,” and “EWSR1-CREM.” References from relevant publications were crossed referenced and yielded further articles. Excluded were cases where the translocation was inconsistently reported in limited numbers of cases of a tumor type and were thus less likely to be defining. The articles reviewed were limited to the English language. Data collected included tumor name, EWSR1 translocation partner, histology, Immunohistochemical (IHC) markers, presence of clear cells, patient demographics and anatomic location were documented, when available.
Results
The PubMed search yielded 162 articles and through title and abstract review, 103 articles were included. A total of 17 neoplasms including CCOC and CCC were described in the 103 articles.
In addition to CCOC and CCC, 6 tumors were found to consistently bear the translocations EWSR1-ATF1, -CREB1, -CREM: angiomatoid fIbrous histiocytoma (AFH), clear cell sarcoma (CCS), clear cell sarcoma-like tumor of the gastrointestinal tract (CCSLTGT), intracranial myxoid mesenchymal tumor (IMMT), malignant gastrointestinal neuroectodermal tumor (M-GNET), and primary pulmonary myxoid sarcoma (PPMS). Similarities in gene fusions as well as IHC markers, anatomic location, presence of clear cells and patient demographics are summarized in Table 1.
Tumor | EWSR1 partner | IHC (+) | IHC (-) | Site | Sex | Age | Clear cells |
---|---|---|---|---|---|---|---|
CCOC | ATF1 | EMA; CK19; AE1/AE3 | Desmin; SMA; Vimentin | Mandible; Maxilla | F | 40s | Present |
CREB1 | |||||||
CREM | |||||||
AFH | ATF1 | ALK; Desmin; CD68; EMA; CD99 | CD34; SMA; S-100 | Limb extremities; Head and neck | F | May-25 | Sometimes present |
CREB1 | |||||||
CREM | |||||||
CCS | ATF1 | S-100; Vimentin; HMB45; MITF; AE1/AE3; Melan-A; PAS | CK19; EMA; SMA; Desmin; CAM5.2 | Whole body | F | 30s | Present |
CREB1 | |||||||
CREM | |||||||
CCC | ATF1 | AE1/AE3; CAM5.2; PAS; EMA; p63 | S-100; SMA; PASD; Calponin | Salivary glands | F | 40s | Present |
M-GNET | ATF1 | S-100; SOX 10; Vimentin | AE1/AE3; SMA; Melan-A; Desmin | Stomach; Intestines | N/A | 40s | Present |
CREB1 | |||||||
CCSLTGT | ATF1 | S-100; SOX10; Vimentin | HMB-45; Melan-A; Tyrosinase; MITF | Stomach; Intestines | N/A | Oct-80 | Sometimes present |
CREB1 | |||||||
IMMT | ATF1 | Desmin; EMA; Vimentin; ALK | AE1/AE3; GFAP; S-100 | Intracranial | N/A | 20s | Absent |
CREB1 | |||||||
CREM | |||||||
PPMS | CREB1 | Vimentin; EMA | SMA; Desmin; ALK | Endobronchial | N/A | 40s | Absent |
Abbrevations: CCOC: Clear Cell Odontogenic Carcinoma; CCC: Clear Cell Carcinoma; IHC: Immunohistochemical; IMMT: Intracranial Myxoid Mesenchymal Tumor; M-GNET: Malignant Gastrointestinal Neuroectodermal Tumor; CCSLTGT: Clear Cell Sarcoma-Like Tumor Of The Gastrointestinal Tract; PPMS: Primary Pulmonary Myxoid Sarcoma; AFH: Angiomatoid FIbrous Histiocytoma
Table 1: Fusion partners, IHC markers, anatomic location, patient demographics and presence of clear cells.
CCOC/CCC and CCS tend to appear in both soft tissue and bone.IMMT and AFH both appear in younger patients, with the median age at diagnosis ranging from the teenage years to the early twenties. CCS, CCOC, CCC, PPMS, CCSLTGT and M-GNET all occur on average later in life around middle age, though cases are reported in a wide range of ages. CCS, CCC, CCOC and AFH all have a slight predilection for females, while no sex predilection was noted for M-GNET, CCSLTGT, IMMT or PPMS (Table 1). Immunohistochemically, there was an appreciable amount of variation among all the listed tumors.
Tumors that inconsistently harbored EWSR1-CREB fusions were angiosarcoma, atypical central neurocytoma, malignant epithelioid tumor of the peritoneal cavity, malignant mesothelioma, neuroendocrine neoplasm, primary intracranial neoplasm, pulmonary mesenchymal tumor, small blue round cell tumor and soft tissue myoepithelial tumor. No further data regarding these tumors was collected.
EWSR1 translocations have been reported in some cases of other tumors but the significance of these findings is unclear.
Discussion
Initial tumor classifications based on histological description have been expanded to include immunohistochemical patterns and, more recently, molecular features detected by a variety of techniques. The first description of a tumor attributed to a chromosomal translocation was in a 1956 paper by Albert Levan characterizing mouse tumors [17,18]. This work was verified and expanded on later in the 1950s and ‘60s by Levan and T.S. Hauschka in Fox Chase, Pennsylvania as well as Peter Nowell and David Hungerford in their work characterizing the Philadelphia chromosome [19,20]. The following decades proved to be fruitful in the search for and characterization of translocated genes in instances of tumor formation18. As genetic descriptions are further developed, tumor classification and nomenclature may change. As tumors become defined and redefined by their genetics, clinical treatments, too, may also be based on genetic translocations and fusion partners, especially within the context of targeted therapies.
Advances in pharmacogenomics have led to specific treatments based on individual genetic information such as in the case of genetically defined breast cancers [21,22]. For example, BRCA1 and BRCA2 are known as regulators of DNA repair, transcription, and cell cycle progression in the context of DNA damage. In addition to the high risk of developing breast cancer for individuals with germline mutations, BRCA1 and BRCA2 are also linked to increased responses to PARP inhibitors. Additionally, in concert with genetic changes found in these tumor cells, a variety of mutations in the genes that are involved in drug metabolism can affect therapeutic responses [21].
Also of interest is that cases exist wherein different tumors with the same mutations respond differently to the same drug therapy. For example, targeting of the BRAF (V600E) oncoprotein with the small- molecule drug PLX4032 (vemurafenib) is a highly effective therapy for treatment of melanoma. However, patients with colon cancer harboring the very same BRAF (V600E) mutation have relatively poor prognosis and demonstrate a lackluster response to this drug, underscoring the imperative of tumor environment and cellular lineage origination [23]. Furthermore, genomic studies have demonstrated that mutations in similar genes, such as ARID1A, PIK3CA and KRAS, are seen in different conditions of varying severity-ovarian clear cell carcinoma and benign endometriosis [24]. Interestingly, these mutations are postulated to be in same cell of origin [25] thus underscoring the importance of tumor environment on treatment efficacy. Therefore, a clearer understanding of what defines tumor behavior is crucial in the delivery of appropriate and effective care. Response of tumors to treatment may be based on the cell lineage from which the malignant cells arise, the environment in which the tumor grows and the genetic alterations (i.e., translocations) present.
In the cases of CCOC and CCC, two rare and intriguing tumors, the presence of an EWSR1 translocation with a fusion partner in the CREB family of proteins seems to be characteristic [6,9-15]. This tumor, which was thought to be benign and locally aggressive, was redefined by the World Health Organization in 2005 as a carcinoma with metastatic potential. It is a malignant neoplasm that may sit dormant for many years and appears to be low grade [16]. Of interest is the fact that some benign tumors of the head and neck can be locally recurrent, aggressive, and destructive (e.g., ameloblastoma and odontogenic keratocysts) [26,27], while some malignancies such as low-grade sarcomas, CCOC, and CCC behave less aggressively than expected of a malignant tumor.
EWSR1 translocations are common among many kinds of mesenchymal tumors and their presence helps to classify tumors such as the Ewing sarcoma family of tumors. These tumors are cytogenetically defined by rearrangements of the EWSR1 gene at 22q12 with FLI1 at 11q24 or ERG at 22q12 in 85% and 5%-10% of cases, respectively. Less than 1% of Ewing’s tumors have other fusion partners such as ETV1 at 7q22, E1AF at 17q12 or FEV at 2q33 [28-31].
Animal models can provide more tissue for study which is of great significance in these rare tumors. They will also allow the study of specific tumors, the cell lineage, genetics, and anatomic location. Understanding these interactions will aid in selecting appropriate treatments since tumor responses are generally multifactorial.
Mouse models with high disease penetrance can mitigate the challenge associated with rare disease therapy development. Straessler, et al. and Komura, et al. separately developed mouse models of CCS [32,33]. In the former model, Cre floxes a loxP-flanked STOP signal, thereby allowing EWSR1-ATF1 expression; in the latter, the EWSR1- ATF1 fusion mutation is expressed under control of the doxycycline- inducible Tet-On system. Both models demonstrated that induction of EWSR1-ATF1 expression in multipotent mesenchymal cells within connective tissues results in malignant transformation into CCS. Studies to further define how similar CCS and CCOC are may be useful. These models may be significant in the investigation of other tumors with EWSR1-CREB fusions such as CCOC and CCC. Future studies will be required to examine how the EWSR1-ATF1 mutation modifies the fate of cells in the region of the head and neck. Furthermore, studies are required to determine exactly which cells are susceptible to undergoing pathologic degeneration to COCC, and whether specific environment/ niche characteristics contribute to this transition in the setting of the EWSR1-ATF1 mutation.
Ongoing study of these seven tumors is currently underway based on immunohistochemistry, cell lineage, environment, and genetic translocations, to further elucidate similarities and differences to CCOC.
Conclusion
EWSR1-CREB fusions are commonly found in 6 tumor types in addition to clear cell odontogenic carcinoma (CCOC) and lear cell carcinoma (CCC) of the salivary gland. The high frequency of EWSR1-CREB fusions suggests that these tumors may share similar oncogenic mechanisms and therefore may be useful as surrogates for the characterization of our tumors of interest: clear cell odontogenic carcinomaand clear cell carcinoma of the salivary gland.
References
- Delattre O, Zucman J, Plougastel B, Desmaze C, Melot T, et al. (1992) Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. Nature 359: 162-165.
[CrossRef], [Google Scholar], [Pubmed]
- Riggi N, Suva ML, Stamenkovic I (2021) Ewing's sarcoma. N Engl J Med 384: 154-164.
[CrossRef]
- De Alava E, Gerald WL (2000) Molecular biology of the Ewing’s sarcoma/primitive neuroectodermal tumor family. J Clin Oncol 18: 204-13.
[CrossRef], [Google Scholar], [Pubmed]
- Jo VY (2020) EWSR1 fusions: Ewing sarcoma and beyond. Cancer Cytopathol 128: 229-231.
- Thway K, Fisher C (2019) Mesenchymal tumors with EWSR1 gene rearrangements. Surg Pathol Clin 12: 165-190.
[CrossRef], [Google Scholar], [Pubmed]
- Bilodeau EA, Weinreb I, Antonescu CR, Zhang L, Dacic S, et al. (2013) Clear cell odontogenic carcinomas show EWSR1 rearrangements: A novel finding and a biological link to salivary clear cell carcinomas. Am J Surg Pathol 37: 1001-5.
[CrossRef], [Google Scholar]
- Weinreb I (2013) Hyalinizing clear cell carcinoma of salivary gland: A review and update. Head Neck Pathol 7: 20-9.
[CrossRef], [Google Scholar], [Pubmed]
- Desai A, Rivera CM, Faquin WC, Iafrate AJ, Rivera MN, et al. (2021) Clear cell carcinoma: A comprehensive literature review of 254 cases. Int J Oral Maxillofac Surg.
[CrossRef], [Google Scholar], [Pubmed]
- Rivera CM, Faquin WC, Thierauf J, Afrogheh AH, Jaquinet A, et al. (2021) Detection of EWSR1 fusions in CCOC by targeted RNA-seq. Oral Surg Oral Med Oral Pathol Oral Radiol.
[CrossRef], [Google Scholar]
- Vogels R, Baumhoer D, Van Gorp J, Eijkelenboom A, Verdijk M, et al. (2019) Clear cell odontogenic carcinoma: Occurrence of EWSR1-CREB1 as alternative fusion gene to EWSR1-ATF1. Head Neck Pathol 13: 225-30.
[CrossRef], [Google Scholar], [Pubmed]
- Kujiraoka S, Tsunematsu T, Sato Y, Yoshida M, Ishikawa A, et al. (2017) Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene. Oral Oncol 69: 46-55.
[CrossRef], [Google Scholar], [Pubmed]
- Ginat DT, Villaflor V, Cipriani NA (2016) Oral cavity clear cell odontogenic carcinoma. Head Neck Pathol 10: 217-20.
[CrossRef], [Google Scholar], [Pubmed]
- Yancoskie AE, Sreekantaiah C, Jacob J, Rosenberg A, Edelman M et al. (2014) EWSR1 and ATF1 rearrangements in clear cell odontogenic carcinoma: Presentation of a case. Oral Surg Oral Med Oral Pathol Oral Radiol 118: e115-8.
[CrossRef], [Google Scholar], [Pubmed]
- Breik O, Higginson J, Al-Ajami AK, Mohamed A, Martin T, et al. (2021) Clear cell odontogenic carcinoma: First report of novel ewsr1–crem fusion gene in case of long-term misdiagnosis. Head Neck Pathol 15: 1391-8.
[CrossRef], [Google Scholar]
- Santana T, De Andrade FL, de Sousa Melo MC, Da Rocha GB, Trierveiler M (2020) Clear cell odontogenic carcinoma harboring the EWSR1–ATF1 fusion gene: Report of a rare case. Head Neck Pathol 14: 847-51.
[CrossRef], [Google Scholar], [Pubmed]
- Guastaldi FP, Faquin WC, Gootkind F, Hashemi S, August M, et al. (2019) Clear cell odontogenic carcinoma: A rare jaw tumor. A summary of 107 reported cases. Int J Oral Maxillofac Surg 48: 1405-10.
[CrossRef], [Google Scholar], [Pubmed]
- Levan A (1956) The Significance of polyploidy for the evolution of mouse tumors: Strains of the TA3 mammary adenocarcinoma with different ploidy. Exp Cell Res 11: 613-29.
[CrossRef], [Google Scholar], [Pubmed]
- Potter M (2008) Brief historical sketch of chromosomal translocations and tumors. J Natl Cancer Inst Monogr 2-7.
[CrossRef], [Google Scholar], [Pubmed]
- Hauschka TS (1958) Correlation of chromosomal and physiologic changes in tumors. J Cell Physiol Suppl 52: 197-233.
[CrossRef ], [Google Scholar]
- Nowell PC, Hungerford DA (1960) Chromosome studies on normal and leukemic human leukocytes. J Cell Physiol Suppl 25: 85-109.
[CrossRef], [Google Scholar], [Pubmed]
- Lima ZS, Ghadamzadeh M, Arashloo FT, Amjad G, Ebadi MR, et al. (2019) Recent advances of therapeutic targets based on the molecular signature in breast cancer: Genetic mutations and implications for current treatment paradigms. J Hematol Oncol 12: 1-25.
[CrossRef], [Google Scholar], [Pubmed]
- Schroth W, Goetz MP, Hamann U, Fasching PA, Schmidt M, et al. (2009) Association between CYP2D6 polymorphisms and outcomes among women with early stage breast cancer treated with tamoxifen. Jama 302: 1429-36.
[CrossRef], [Google Scholar], [Pubmed]
- Prahallad A, Sun C, Huang S, Di Nicolantonio F, Salazar R, et al. (2012) Unresponsiveness of colon cancer to BRAF (V600E) inhibition through feedback activation of EGFR. Nature 483: 100-3.
[CrossRef], [Google Scholar], [Pubmed]
- Suda K, Cruz Diaz LA, Yoshihara K, Nakaoka H, Yachida N, et al. (2020) Clonal lineage from normal endometrium to ovarian clear cell carcinoma through ovarian endometriosis. Cancer Sci 111: 3000-9.
[CrossRef], [Google Scholar], [Pubmed]
- Kolin DL, Dinulescu DM, Crum CP (2018) Origin of clear cell carcinoma: Nature or nurture? J Pathol 244: 131-34.
[CrossRef], [Google Scholar], [Pubmed]
- Milman T, Ying GS, Pan W, LiVolsi V (2016) Ameloblastoma: 25 year experience at a single institution. Head Neck Pathol 10: 513-20.
[CrossRef], [Google Scholar], [Pubmed]
- Shear M (2002) The aggressive nature of the odontogenic keratocyst: Is it a benign cystic neoplasm? Part 3. Immunocytochemistry of cytokeratin and other epithelial cell markers. Oral Oncol 38: 407-15.
[CrossRef], [Google Scholar] [Pubmed]
- Paronetto MP (2013) Ewing sarcoma protein: A key player in human cancer. Int J Cell Biol. [CrossRef], [Google Scholar], [Pubmed]
- Plougastel B, Zucman J, Peter M, Thomas G, Delattre O (1993) Genomic structure of the EWS gene and its relationship to EWSR1, a site of tumor-associated chromosome translocation. Genomics 18: 609-15.
[CrossRef], [Google Scholar], [Pubmed]
- Romeo S, Dei Tos AP (2010) Soft tissue tumors associated with EWSR1 translocation. Virchows Arch 456: 219-34.
- Riley RD, Burchill SA, Abrams KR, Heney D, Sutton AJ, et al. (2003) A systematic review of molecular and biological markers in tumours of the ewing's sarcoma family. Eur J Cancer 39: 19-30.
[CrossRef], [Google Scholar], [Pubmed]
- Straessler KM, Jones KB, Hu H, Jin H, Van de Rijn M, et al. (2013) Modeling clear cell sarcomagenesis in the mouse: Cell of origin differentiation state impacts tumor characteristics. Cancer Cell 23: 215-27.
[CrossRef], [Google Scholar], [Pubmed]
- Komura S, Ito K, Ohta S, Ukai T, Kabata M, et al. (2019) Cell-type dependent enhancer binding of the EWS/ATF1 fusion gene in clear cell sarcomas. Nat Commun 10: 1-9.
[CrossRef], [Google Scholar], [Pubmed]
Citation: Weil SG, Rivera CM, Thierauf JC, Faquin WC, Choy E, et al. (2022) Occurrence and Summary of Literature Documenting Tumors with EWSR1 and CREB Family Transcription Factor Fusions. J Clin Exp Pathol 12: 407. DOI: 10.4172/2161-0681.1000407
Copyright: © 2022 Weil SG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Share This Article
Recommended Journals
Open Access Journals
Article Tools
Article Usage
- Total views: 1877
- [From(publication date): 0-2022 - Aug 16, 2024]
- Breakdown by view type
- HTML page views: 1522
- PDF downloads: 355