Isolation & Characterization of Arsenic-Resistant Bacteria and In Bioremediation
Received: 01-Jun-2022 / Manuscript No. jbrbd-22- 71043 / Editor assigned: 03-Jun-2022 / PreQC No. jbrbd-22- 71043 (PQ) / Reviewed: 17-Jun-2022 / QC No. jbrbd-22-71043 / Revised: 22-Jun-2022 / Manuscript No. jbrbd-22- 71043 (R) / Accepted Date: 29-Jun-2022 / Published Date: 29-Jun-2022 DOI: 10.4172/2155-6199.1000517
Abstract
Arsenic contamination of ground water is a major issue in many developing nations, including Bangladesh and India. The development of cutting-edge, modern technology for removing arsenic from aqueous systems has recently attracted research interest. In the current study, two rod-shaped Gram-positive bacteria that can withstand arsenate concentrations up to ppm and ppm of arsenite concentration are reported. These bacteria were isolated from ground water that had been contaminated with arsenic in the Purbasthali block of Burdwan, West Bengal, India they were identified as Bacillus sp.
Keywords: Arsenic contamination, Anaemia, Gram-positive bacteria
Editorial
Arsenic contamination of ground water is a major issue in many developing nations, including Bangladesh and India [1]. The development of cutting-edge, modern technology for removing arsenic from aqueous systems has recently attracted research interest. In the current study, two rod-shaped Gram-positive bacteria that can withstand arsenate concentrations up to ppm and ppm of arsenite concentration are reported. These bacteria were isolated from ground water that had been contaminated with arsenic in the Purbasthali block of Burdwan, West Bengal, India they were identified as Bacillus sp [2]. And Aneurinibacillus aneurinilyticus, respectively, using biochemical investigation and sequencing. The isolates and can, respectively, eliminate and of arsenite and and of arsenate from culture media containing arsenic. Each individual isolation can convert toxic arsenite to less hazardous arsenate. A unique method for the bioremediation of arsenic can be created using these two arsenic-resistant bacteria. Elsevier B.V. is the publisher [3]. The CC BY-NC-ND licence governs this open access article. Insoluble sulphides and sulfosalts of arsenic, including arsenopyrite, orpiment, realgar, lollingite, and tennantite, are found in soil and are hazardous metalloid arsenic. Despite arsenic's presence in the earth's crust, arsenic contamination is primarily the result of human activity, such as the excessive use of arsenic in pesticides, herbicides, wood preservatives, and pharmaceuticals [4]. Arsine elemental arsenic, arsenite, and arsenat are the primary forms of arsenic that are found in the environment. Only two of these types, arsenite and arsenate, are more prevalent in the natural world than the others. Health impacts of toxins. Skin itchiness, skin cancer, weight loss, appetite loss, weakness, lethargy, and easily becoming exhausted are the most typical symptoms of arsenic toxicity. Other symptoms include chronic respiratory disorders, gastrointestinal disorders like anorexia, nausea, abdominal pain, enlarged liver and spleen, and moderate to severe anaemia was also reported in a few cases [5]. Arsenic is extremely difficult to remove from contaminated water due to its high magnitude of solubility. Arsenic can be removed from drinking water using a variety of standard techniques, including coagulation, membrane filtration, reverse osmosis, adsorption, filtration, etc. The oxidation phase needed for the conversion of As to As in these traditional procedures is obtained either through the reaction with oxygen under normal [6]. Through chemical oxidants like hydrogen peroxide, chlorine, and ozone, which are very expensive and also produce toxic by-products, or through atmospheric conditions, which are very slowly. By utilising the pollutants as energy sources during their metabolic processes, microorganisms might lessen the toxicity of the contaminants. Arsenite methylation, arsenite oxidation, and other methods have been created by microorganisms to convert arsenite, a more poisonous form of arsenic, into arsenate, a less deadly form. Arsenic oxidase is a unique type of enzyme found in the protoplasm of bacteria that oxidise arsenic. The bacteria convert arsenite to arsenate by using this enzyme. There are numerous arsenic-resistant microorganisms that can endure high arsenic concentrations and may be useful. For the bioremediation of arsenic from ground water that has been contaminated by arsenic. From an arsenic-contaminated borewell in West Bengal, India, Chowdhury et al. discovered an unique strain of Planococcus called KRPC10YT that can withstand up to 30 mM of arsenate and 20 mM of arsenite. Bacillus arsenicus, and unique arsenic-resistant strain, was discovered by Shivaji et al. in from soils contaminated with arsenic in the Chakdah region of West Bengal, India. This strain could thrive in the presence of mM arsenate and mM arsenite. However, very little has been done to bioremediate arsenic utilising bacteria resistant to arsenic. Arsenic contamination in the Purbasthali block of Burdwan, West Bengal, India, is severe. According to Roy et al., this region's tubewell water has an arsenic content of ppm. But until now, no studies have been done to identify arsenicresistant bacteria from this specific affected area or to use these bacteria to bioremediate arsenic-contaminated ground water. In the current study, two arsenic resistant bacteria are reported that can reduce the concentration of arsenic in contaminated water and are resistant to very high concentrations of arsenate and arsenite from the arseniccontaminated water of Purbasthali, Misbahpur Paschim Atpara, Purba Atpara, Kamalnagar, and Laxmipur Natun. Rukuspur, Sinhari Tamaghata Majida, and Laxmipur Dhamas . Water samples were collected, and in-situ measurements of pH, electrical conductivity, and total dissolved solids
were made using multi-parameter equipment. Following industry standard procedures, other parameters including acidity, alkalinity, hardness, chloride, phosphate, and iron were assessed.
Acknowledgement
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Conflict of Interest
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Citation: Dey U (2022) Isolation & Characterization of Arsenic-Resistant Bacteria and in Bioremediation. J Bioremediat Biodegrad, 13: 517. DOI: 10.4172/2155-6199.1000517
Copyright: © 2022 Dey U. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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